p21WAF1 (DCS-60.2) Mouse Monoclonal Antibody
SIGMA/421M-1
Product Type: Chemical
Immunohistochemistry p21WAF1 (DCS-60.2) on colon tissue
| antibody form | diluted ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | DCS-60.2, monoclonal |
| conjugate | unconjugated |
| control | colon |
| description | For In Vitro Diagnostic Use in Select Regions (See Chart) |
| form | buffered aqueous solution |
| isotype | IgG2a |
| manufacturer/tradename | Cell Marque® |
| packaging | bottle of 1.0 mL predilute (421M-17) |
| bottle of 7.0 mL predilute (421M-18) | |
| vial of 0.1 mL concentrate (421M-14) | |
| vial of 0.5 mL concentrate (421M-15) | |
| vial of 1.0 mL concentrate (421M-16) | |
| Quality Level | 100  |
| 500 |
|
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:200 |
| visualization | nuclear |
| Analysis Note: |
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| General description: | p21 is a nuclear 21 kD protein, a product of the WAF1/CIP1 gene. It is a cyclin-dependent kinase inhibitor 1A (p21, Cip1), also known as CDKN1A, which in humans is encoded by the CDKN1A gene located on chromosome (6p21.2). It is a constituent of a large complex of nuclear proteins, including cyclins, cyclin dependent kinases, and PCNA. Cell cycle progression is regulated by cyclins and their cognate Cdks. p21 inhibits the activity of each member of the cyclin/Cdk family. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma). | ||||
| Legal Information: | Cell Marque is a registered trademark of Merck KGaA, Darmstadt, Germany | ||||
| Other Notes: | For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com | ||||
| Other Notes: | p21WAF1 Positive Control Slides, Product No. 421S , are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections). |
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| Physical form: | Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide | ||||
| Preparation Note: | Download the IFU specific to your product lot and format  Note: This requires a keycode which can be found on your packaging or product label. |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |