DL-Dithiothreitol solution

SIGMA/43816 - BioUltra, Molecular Biology, ~1 M in H₂O

Synonym: Cleland′s reagent, (±)-Dithiothreitol, rac-Dithiothreitol, Dithiothreitol; (±)-threo-1,4-Dimercapto-2,3-butanediol solution; DTT

CAS Number: 3483-12-3
Empirical Formula (Hill Notation): C₄H₁₀O₂S₂
Molecular Weight: 154.25
MDL Number: MFCD00004877
Linear Formula: C₄H₁₀O₂S₂
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-43816-10ML	10 mL	$48.20 1/EA	Add To Favorites
45-43816-50ML	50 mL	$144.00 1/EA	Add To Favorites
45-43816-250ML	250 mL	$571.00 1/EA	Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

absorption	cut-off at 330 nm
assay	900-1100 mmol/L (Titration molarity)
concentration	~1 M in H₂O
density	1.04 g/mL at 20 °C
form	liquid
grade	Molecular Biology
impurities	DNases, none detected
	phosphatases, none detected
	proteases, none detected
	RNases, none detected
InChI	1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
InChI key	VHJLVAABSRFDPM-QWWZWVQMSA-N
pH	2.7-5.0
product line	BioUltra
Quality Level	200
reaction suitability	reagent type: reductant
SMILES string	O[C@H](CS)[C@H](O)CS
storage temp.	2-8°C
suitability	in accordance for filter test
	suitable for molecular biology

Application:	DL-dithiothreitol solution has been used: • in the treatment of intestinal explants • in homogenate preparation of gill cells for enzymatic assays • in protein electrophoresis for western blotting • in transglutaminase activity • in sample buffer for SDS-PAGE • in Western Blot, as a lysis buffer component for single-nucleus RNA-sequencing • as a component of protein extraction buffer for cell lysis
Biochem/physiol Actions:	DL-dithiothreitol (DTT) is used in a chemical debonding technique, that helps to remove bacteria from biofilms. It can change the structural stability of the GABA(B) (gamma-aminobutyric acid) receptor by disabling the disulfide bonds between four cysteine residues positioned in the GABA(B1(a)) receptor structure.Dithiothreitol (DTT) is broadly utilized in synthetic peptide synthesis and biochemical arrangements of thiol proteins. It can also be used to study protein chemistry topics like enzyme activity and protein folding. DTT explicitly controls the thiol-disulfide exchange response to completely reduce the intra-or between sub-atomic disulfide bonds in biomolecules. Thiols and the cyclic disulfide of DTT are the products of this reaction.
Features and Benefits:	• BioUltra grade - 1M DTT solution, suitable for molecular biology • Free from DNases, RNases, phosphatases, and proteases
General description:	Dithiothreitol (DTT), a thiol group protectant, is an excellent reagent for preventing the oxidation of SH groups in proteins. DTT solution is a potent and versatile disulfide-reducing agent. It reduces a disulfide bond by forming a six-membrane ring and initiating a thiol-disulfide interchange.
Other Notes:	For additional information on our range of Biochemicals , please complete this form .

Symbol	GHS05
Signal word	Danger
Hazard statements	H315 - H318
Precautionary statements	P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P362 + P364
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	900-1100 mmol/L (Titration molarity)
Density	1.04 g/mL at 20 °C
Storage Temp.	2-8°C
UNSPSC	12352201

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