iTRAQ® Reagent - 8PLEX Multiplex Kit
SIGMA/4381663
Product Type: Chemical
Quality Level | 100 |
storage temp. | −20°C |
Analysis Note: | To view the Protocol for the iTRAQ Reagents 8-plex Kit, please visit this Protocol link . To view the Chemistry Quick Reference Card for the iTRAQ Reagents, please visit this Chemistry Quick Reference Card link . |
Application: | iTRAQ® Reagent - 8PLEX Multiplex Kit has been used: • to derivatize and label the tryptic digests of the iodoacetamide probe (IPM)-labeled HeLa proteome • in isobaric tags for relative and absolute quantitation (iTRAQ) labeling of peptides • as isobaric labels for the comparative quantification of proteins between the digested samples in proteomic analysis |
Features and Benefits: | • Simultaneously identify and quantify proteins from multiple samples • Expands protein and proteome coverage by labeling all peptides, including those with post-translational modifications (PTMs) • Increases confidence in identification and quantitation from MS/MS spectra by tagging multiple peptides per protein • Increases throughput and confidence in results for protein biomarker discovery studies • Offers a simple workflow without sample fractionation for reduced-complexity samples, such as affinity pull-downs • Provides the flexibility to multiplex up to eight different biological samples simultaneously in a single experiment • Fully supported by ProteinPilot Software on all SCIEX proteomics LC/MS/MS platforms |
General description: | Contains iTRAQ® Reagents 113, 114, 115, 116, 117, 118, 119 and 121 and sufficient material for five 8-plex assays. Multiplex Buffer kit (Cat. No. 4381664) to be ordered separately. Cat. No. 4381663 contains the equivalent reagents in the Sciex iChemistry® product number 4390812. |
General description: | The isobaric tags for relative and absolute quantitation (iTRAQ®) reagents are the first set of multiplexed, amine-specific, stable-isotope reagents that can label all peptides in up to eight different biological samples enabling simultaneous identification and quantitation, relative and absolute, while retaining important post-translational modification (PTM) information. The iTRAQ Reagents - 8plex kits are available in more economical bulk packs. |
Legal Information: | iChemistry is a registered trademark of AB Sciex Pte. Ltd. |
Legal Information: | iTraq is a registered trademark of AB Sciex Pte. Ltd. |
Symbol | GHS02,GHS07 |
Signal word | Danger |
Hazard statements | H225 - H302 + H312 + H332 - H319 |
Precautionary statements | P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P312 - P305 + P351 + P338 |
Hazard Codes | F,Xn |
Risk Statements | 11-20/21/22-36 |
Safety Statements | 16-26-36/37 |
RIDADR | UN 3316 9 |
WGK Germany | WGK 3 |
Flash Point(F) | 35.6 °F - closed cup |
Flash Point(C) | 2.0 °C - closed cup |
Storage Temp. | −20°C |
UNSPSC | 12352200 |