DL-Dithiothreitol
SIGMA/43819 - ≥99.0% (RT)
Synonym: threo-
CAS Number: 3483-12-3
Empirical Formula (Hill Notation): C4H10O2S2
Molecular Weight: 154.25
EC Number: 222-468-7
MDL Number: MFCD00004877
Linear Formula: HSCH2CH(OH)CH(OH)CH2SH
Product Type: Chemical
| anion traces | sulfate (SO42-): ≤50 mg/kg |
| assay | ≥99.0% (RT) |
| cation traces | Ca: ≤10 mg/kg |
| Cd: ≤5 mg/kg | |
| Co: ≤5 mg/kg | |
| Cr: ≤5 mg/kg | |
| Cu: ≤5 mg/kg | |
| Fe: ≤5 mg/kg | |
| K: ≤50 mg/kg | |
| Mg: ≤5 mg/kg | |
| Mn: ≤5 mg/kg | |
| Na: ≤250 mg/kg | |
| Ni: ≤5 mg/kg | |
| Pb: ≤5 mg/kg | |
| Zn: ≤5 mg/kg | |
| form | powder |
| impurities | ≤0.3% 4,5-dihydroxy-1,2-dithian |
| InChI | 1S/C4H10O2S2/c5-3(1-7)4(6 |
| InChI key | VHJLVAABSRFDPM-QWWZWVQMSA |
| mp | 40-43 °C |
| 41-44 °C (lit.) | |
| Quality Level | 200  |
| reaction suitability | reagent type: reductant |
| SMILES string | O[C@H](CS)[C@H](O)CS |
| solubility | acetone: freely soluble |
| chloroform: freely soluble | |
| diethyl ether: freely soluble | |
| ethanol: freely soluble | |
| ethyl acetate: freely soluble | |
| H2O: 50 mg/mL, clear, colorless | |
| H2O: freely soluble | |
| storage temp. | 2-8°C |
| Application: | An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystami |
| Application: | DL-Dithiothreitol is a pivotal component used in the preparation of buffer which in turn is utilized in the platelet cytosol preparation. It is also important in isolation of RNA by using guanidinium salts and homogenisation of tissue and cells. |
| Biochem/physiol Actions: | DL-Dithiothreitol denatures proteins by reducing the disulphide bonds and reducing them to SH groups. It also prevents intramolecular and intermolecular disulfide bond formation between cysteine residues of proteins. |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research  |
| Symbol |   GHS05,GHS07 |
| Signal word | Danger |
| Hazard statements | H302 - H315 - H318 |
| Precautionary statements | P264 - P270 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 22-36/37/38 |
| Safety Statements | 26-36 |
| RIDADR | UN 3335 |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99.0% (RT) |
| mp | 40-43 °C; 41-44 °C (lit.) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352201 |