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Wright Stain solution

SIGMA/45253 - for microscopy

Synonym: Eosin Methylene blue solution according to Wright; Wright’s eosin methylene blue

MDL Number: MFCD00005038
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-45253-250ML-F 250 mL
$47.10
1/EA
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45-45253-1L-F 1 L
$152.00
1/EA
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density 0.80 g/mL at 20 °C
grade for microscopy
impurities methanol
InChI 1S/C21H10Br4O5.K/c1-29-21(28)9-5-3-2-4-8(9)14-10-6-12(22)17(26)15(24)19(10)30-20-11(14)7-13(23)18(27)16(20)25;/h2-7,26H,1H3;/q;+1/p-1
InChI key NXBZXYJXNIFCRJ-UHFFFAOYSA-M
Quality Level 100 
shelf life limited shelf life, expiry date on the label
SMILES string [K+].COC(=O)c1ccccc1C2=C3C=C(Br)C(=O)C(Br)=C3Oc4c(Br)c([O-])c(Br)cc24
suitability Chlamydia spp.
  Histoplasma spp.
  Plasmodium spp.
  Trichomonas spp.
  bacteria
  protozoa
  spirochetes
technique(s) microbe id | staining: suitable
Application: Wright stain has been used to determine the number of white blood cell types (neutrophils, lymphocytes, monocytes, eosinophils, and basophils) per microliter of whole blood.
General description: Wright Stain is a modified Romanowsky stain commonly used for the cellular elements of blood. Wright’s stain is a mixture of eosin Y with polychromed (oxidized and partly demethylated) methylene blue and therefore termed polychrome stains. The hematological stain is intended for differentially staining peripheral blood and bone marrow films.
Other Notes: Blood stain
Density 0.80 g/mL at 20 °C
UNSPSC 12171500

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