Ethidium homodimer

SIGMA/46043 - suitable for fluorescence, ~90% (HPCE)

Synonym: EtDi; EthD-1

CAS Number: 61926-22-5
Empirical Formula (Hill Notation): C₄₆H₅₀Cl₄N₈
Molecular Weight: 856.75
EC Number: 263-325-9
MDL Number: MFCD00210948
Linear Formula: C₄₆H₅₀Cl₄N₈
Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-46043-1MG-F	1 mg	$406.00 1/EA		Add To Favorites
45-46043-5MG-F	5 mg	$1640.00 1/EA		Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

assay	~90% (HPCE)
fluorescence	λ_ex 493 nm in H₂O
	λ_ex 528 nm; λ_em 617 nm in TE buffer; DNA
form	solid
InChI	1S/C46H46N8.4ClH/c47-33-13-17-37-39-19-15-35(49)29-43(39)53(45(41(37)27-33)31-9-3-1-4-10-31)25-7-21-51-23-24-52-22-8-26-54-44-30-36(50)16-20-40(44)38-18-14-34(48)28-42(38)46(54)32-11-5-2-6-12-32;;;;/h1-6,9-20,27-30,49-52H,7-8,21-26,47-48H2;4*1H
InChI key	ZKAJUKABUUJRLB-UHFFFAOYSA-N
mp	≥250 °C (lit.)
Quality Level	100
SMILES string	Cl.Cl.[Cl-].[Cl-].Nc1ccc2c(c1)c(-c3ccccc3)[n+](CCCNCCNCCC[n+]4c(-c5ccccc5)c6cc(N)ccc6c7ccc(N)cc47)c8cc(N)ccc28
solubility	DMF: soluble
	DMSO: soluble
	H₂O: soluble
storage temp.	−20°C
suitability	suitable for fluorescence

Application:	Ethidium homodimer (EtDi, EthD-1) is used as a staining dye for ssDNA, dsDNA, RNA, oligonucleotides, and triplex DNA. EthD-1 does not cross intact/viable cell membranes and can be used to test cell viability.
Application:	Staining dye for ssDNA, dsDNA, RNA, oligonucleotides, and triplex DNA. It will not cross intact cell membranes and can be used to test cell viability.
Other Notes:	Reagent for the fluorimetric detection of nucleic acids
Packaging:	Bottomless glass bottle. Contents are inside inserted fused cone.

Symbol	GHS02,GHS05,GHS08
Signal word	Danger
Hazard statements	H261 - H314 - H351
Precautionary statements	P201 - P231 + P232 - P260 - P280 - P303 + P361 + P353 - P305 + P351 + P338 + P310
Hazard Codes	C
Risk Statements	14-34-40
Safety Statements	26-36/37/39-45
RIDADR	UN 3096 4.3(8) / PGII
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	~90% (HPCE)
mp	≥250 °C (lit.)
Storage Temp.	−20°C
UNSPSC	12161505

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