Synonym: 4-(2,7-Dichloro-6-hydroxy-3-oxo-9-xanthenyl)-4′-methyl-2,2′-(ethylenedioxy)dianiline-N,N,N′,N′-tetraacetic acid
CAS Number: 123632-39-3
Empirical Formula (Hill Notation): C36H30Cl2N2O13
Molecular Weight: 769.54
MDL Number: MFCD00083337
Linear Formula: C36H30Cl2N2O13
Product Type: Chemical
| assay |
~70% |
| fluorescence |
λex 506 nm; λem 526 nm in 0.1 M Tris pH 8.0, esterase 30 mM Ca2+ |
| form |
solid |
| InChI |
1S/C36H30Cl2N2O13/c1-18-2-4-24(39(14-32(43)44)15-33(45)46)30(8-18)51-6-7-52-31-9-19(3-5-25(31)40(16-34(47)48)17-35(49)50)36-20-10-22(37)26(41)12-28(20)53-29-13-27(42)23(38)11-21(29)36/h2-5,8-13,41H,6-7,14-17H2,1H3,(H,43,44)(H,45,46)(H,47,48)(H,49,50) |
| InChI key |
OZLGRUXZXMRXGP-UHFFFAOYSA-N |
| Quality Level |
100  |
| SMILES string |
Cc1ccc(N(CC(O)=O)CC(O)=O)c(OCCOc2cc(ccc2N(CC(O)=O)CC(O)=O)C3=C4C=C(Cl)C(=O)C=C4Oc5cc(O)c(Cl)cc35)c1 |
| solubility |
H2O: soluble |
| storage temp. |
−20°C |
| suitability |
suitable for fluorescence |
| Application: |
Fluo-3 and related molecule Fluo3/AM are used as a fluorescence indicator of intracellular calcium (Ca2+). Fluo-3 may be use for flow cytometry and confocal laser scanning microscopy using visible light excitation (compatible with argon laser sources operating at 488 nm). Fluorescence intensity increases about 40-fold after calcium binding. |
| Other Notes: |
Fluorescent indicator for calcium used in flow cytometry. Fluorescence intensity increases about 40-fold after calcium binding |
| RIDADR |
NONH for all modes of transport |
| WGK Germany |
WGK 3 |
| Flash Point(F) |
Not applicable |
| Flash Point(C) |
Not applicable |
| Purity |
~70% |
| Storage Temp. |
−20°C |
| UNSPSC |
12352200 |
