6-[Fluorescein-5(6)-carboxamido]hexanoic acid N-hydroxysuccinimide ester

SIGMA/46940 - suitable for fluorescence, ≥75% (HPLC)

Synonym: Fluorescein-5(6)-carboxamidocaproic acid N-succinimidyl ester

CAS Number: 114616-31-8
Empirical Formula (Hill Notation): C₃₁H₂₆N₂O₁₀
Molecular Weight: 586.55
MDL Number: MFCD00467542
Linear Formula: C₃₁H₂₆N₂O₁₀
Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-46940-5MG-F	5 mg	$274.00 1/EA		Add To Favorites
45-46940-25MG-F	25 mg	$851.00 1/EA		Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

assay	≥75% (HPLC)
fluorescence	λ_ex 490 nm; λ_em 514 nm (pH 8.2)
	λ_ex 491 nm; λ_em 515 nm in 0.1 M Tris pH 9.0
form	solid
InChI	1S/2C31H26N2O10/c34-18-6-9-22-24(15-18)41-25-16-19(35)7-10-23(25)31(22)21-8-5-17(14-20(21)30(40)42-31)29(39)32-13-3-1-2-4-28(38)43-33-26(36)11-12-27(33)37;34-18-6-9-21-24(15-18)41-25-16-19(35)7-10-22(25)31(21)23-14-17(5-8-20(23)30(40)42-31)29(39)32-13-3-1-2-4-28(38)43-33-26(36)11-12-27(33)37/h2*5-10,14-16,34-35H,1-4,11-13H2,(H,32,39)
InChI key	WAGYELYAQOYZFU-UHFFFAOYSA-N
Quality Level	100
SMILES string	Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)C(=O)NCCCCCC(=O)ON6C(=O)CCC6=O)c1.Oc7ccc8c(Oc9cc(O)ccc9C8%10OC(=O)c%11ccc(cc%10%11)C(=O)NCCCCCC(=O)ON%12C(=O)CCC%12=O)c7
solubility	DMF: soluble
	methanol: soluble
storage temp.	−20°C
suitability	suitable for fluorescence

Application:	6-[Fluorescein-5(6)-carboxamido] hexanoic acid N-hydroxysuccinimide ester (FHS) is used as a reagent to conjugate the fluorophore 6-[Fluorescein-5(6)-carboxamido] hexanoic acid to molecules.6-[Fluorescein-5(6)-carboxamido] hexanoic acid is used as a fluorescein-based fluorescent label for proteins that can be analyzed in applications such as quenching experiments.
Application:	Reagent for introducing the fluorescein label; the spacer renders the reactive group more accessible to nucleophiles on biopolymers
General description:	6-[Fluorescein-5(6)-carboxamido] hexanoic acid N-hydroxysuccinimide ester (FNHS) is a fluorescent label, also known as fluorescein. FNHS max. excitation is at 490 nm with emission max. at 514nm. 6-[Fluorescein-5(6)-carboxamido] hexanoic acid N-hydroxysuccinimide ester binds covalently with primary amines.

Symbol	GHS07
Signal word	Warning
Hazard statements	H315 - H319 - H335
Precautionary statements	P302 + P352 - P305 + P351 + P338
Hazard Codes	Xi
Risk Statements	36/37/38
Safety Statements	26-36
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥75% (HPLC)
Storage Temp.	−20°C
UNSPSC	12352108

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