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Fluorescein 5(6)-isothiocyanate

SIGMA/46950 - BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)

Synonym: FITC; Fluorescein isothiocyanate; FITC; Fluorescein isothiocyanate

CAS Number: 27072-45-3
Empirical Formula (Hill Notation): C21H11NO5S
Molecular Weight: 389.38
EC Number: 248-207-7
MDL Number: MFCD00005063
Linear Formula: C21H11NO5S
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-46950-50MG-F 50 mg
 $42.90
1/EA		 Add To Favorites
45-46950-250MG-F 250 mg
 $152.00
1/EA		 Add To Favorites
45-46950-1G-F 1 g
 $426.00
1/EA		 Add To Favorites

 

assay ≥90% (HPLC)
fluorescence λex 492 nm; λem 518 nm in 0.1 M phosphate pH 8.0
InChI 1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
InChI key MHMNJMPURVTYEJ-UHFFFAOYSA-N
product line BioReagent
Quality Level 100 
SMILES string Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1
storage temp. −20°C
suitability suitable for fluorescence
Application: Fluorescein 5(6)-isothiocyanate (FITC) labelled molecules are used in non-microscopic immunoassays and physiological tracing experiments. FITC is widely used in flow cytometry experiments for staining proteins, nuclei, and subnuclear particles. White blood cells (WBCs) are marked with FITC to study their response toward a chemoattractant, eventually being measured.
Application: For fluorescent labeling of proteins (e.g. immunofluorescence); H Protein labeling for gel chromatography
General description: Fluorescein 5(6)-isothiocyanate, also known as FITC, is a weakly acidic hydroxyxanthene whose overall size is small, with both carboxylic acid and isothiocyanate substituents on the pendant phenyl ring. The dye has a moderately sized, nonplanar aromatic system. The isothiocyanate group reacts with nucleophilic substituents (e.g. amino, hydroxyl, thiol) of biomolecules to be attached as a fluorescent label. The maximum emission of the dye is at 519nm in the same solvent.
Packaging: Bottomless glass bottle. Contents are inside inserted fused cone.
Hazard Codes Xn
Risk Statements 42
Safety Statements 22-24/25
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Purity ≥90% (HPLC)
Storage Temp. −20°C
UNSPSC 12352200

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