Fluorescein
SIGMA/46955 - for fluorescence, free acid
Synonym: Acid Yellow 73
CAS Number: 2321-07-5
Empirical Formula (Hill Notation): C20H12O5
Molecular Weight: 332.31
EC Number: 219-031-8
MDL Number: MFCD00005050
Linear Formula: C20H12O5
Product Type: Chemical
| fluorescence | λex 490 nm; λem 514 nm in 0.1 M Tris pH 8.0 |
| grade | for fluorescence |
| InChI | 1S/C20H12O5/c21-11-5-7-15 |
| InChI key | GNBHRKFJIUUOQI-UHFFFAOYSA |
| Quality Level | 100  |
| SMILES string | Oc1ccc2c(Oc3cc(O)ccc3C24O |
| suitability | suitable for fluorescence |
| technique(s) | titration: suitable |
| Application: | Fluorescein has been used: • in the permeability assay of Caco-2 cells • to test its suitability to serve as lifetime fluorescence standards • as a fluorophore for the fast recovery of fluorescence peaks and fluorophore concentration in the presence of hemoglobin • as a fluorescent probe in oxygen radical absorbance capacity (ORAC) assay |
| General description: | Fluorescein dye is the most commonly used fluorescent probe. It is used to stain epithelial defects and analyse the integrity of corneal epithelium. Fluorescein is used in the diagnosis of corneal perforation. Oral or intravenous fluorescein is used in fluorescein angiography to detect diabetic retinopathy and choroidal neovascular membrane. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H319 |
| Precautionary statements | P264 - P280 - P305 + P351 + P338 - P337 + P313 |
| Hazard Codes | Xi |
| Risk Statements | 36 |
| Safety Statements | 26 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Colour Index Number | 45350 |
| UNSPSC | 12171500 |