Formaldehyde solution
SIGMA/47608 - for molecular biology, BioReagent, ≥36.0% in H2O (T)
Synonym: Formic aldehyde; Formol; Formalin
CAS Number: 50-00-0
Empirical Formula (Hill Notation): CH2O
Molecular Weight: 30.03
MDL Number: MFCD00003274
Linear Formula: HCHO
Product Type: Chemical
| λ | 2 M in H2O |
| anion traces | chloride (Cl-): ≤10 mg/kg |
| sulfate (SO42-): ≤20 mg/kg | |
| autoignition temp. | 572 °F |
| cation traces | Ca: ≤5 mg/kg |
| Cd: ≤1 mg/kg | |
| Co: ≤1 mg/kg | |
| Cr: ≤1 mg/kg | |
| Cu: ≤1 mg/kg | |
| Fe: ≤1 mg/kg | |
| K: ≤20 mg/kg | |
| Mg: ≤1 mg/kg | |
| Mn: ≤1 mg/kg | |
| Na: ≤100 mg/kg | |
| Ni: ≤1 mg/kg | |
| Pb: ≤1 mg/kg | |
| Zn: ≤1 mg/mL | |
| concentration | ≥36.0% in H2O (T) |
| 36.0-38.0% (aldehyde method) | |
| contains | ~10% methanol as stabilizer |
| density | 1.09 g/mL at 20 °C |
| 1.09 g/mL at 25 °C (lit.) | |
| form | liquid |
| grade | for molecular biology |
| ign. residue | ≤0.05% (as SO4) |
| impurities | ≤0.03% free acid (as HCOOH) |
| InChI | 1S/CH2O/c1-2/h1H2 |
| InChI key | WSFSSNUMVMOOMR-UHFFFAOYSA |
| pH | 2.8-4.0 |
| product line | BioReagent |
| Quality Level | 100  |
| SMILES string | [H]C([H])=O |
| storage temp. | room temp |
| suitability | in accordance for electrophoresis of RNA in agarose gels |
| technique(s) | electrophoresis: suitable |
| UV absorption | λ: 260 nm Amax: 0.01 |
| λ: 280 nm Amax: 0.01 | |
| vapor density | 1.03 (vs air) |
| vapor pressure | 52 mmHg ( 37 °C) |
| Application: | Formaldehyde solution is suitable for:• denaturation and separation of RNA molecules • anti-bacterial treatment • fixation of cells Formaldehydesolution has been used:• in the fixation of human spermatogonial stem cell transplantation (SSCT). • to crosslink T- cell acute lymphoblastic leukemia protein/DNA complex during chromatin immunoprecipitation. • in fixing zebra fish larvae for neutrophil staining. |
| Caution: | may contain precipitate (paraformaldehyde) |
| General description: | Formaldehyde is a molecular biology reagent that denaturates RNA during electrophoresis when incorporated in agarose gels. It is an aliphatic aldehyde that is widely used in industrial applications for the synthesis of several chemicals such as melamine formaldehyde, urea-formaldehyde, phenol formaldehyde and industrial resins, paraformaldehyde, polyols, chelating agents, slow release fertilizers, 1,4-butanediol and hexamethylenetetramine. |
| Principle: | Formaldehyde disrupts the secondary structure of RNA and allows separation and identification of RNA species based on charge migration. |
| Symbol |     GHS02,GHS05,GHS06,GHS08 |
| Signal word | Danger |
| Hazard statements | H226 - H301 + H311 - H314 - H317 - H330 - H335 - H341 - H350 - H370 |
| Precautionary statements | P201 - P210 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 |
| Hazard Codes | T |
| Risk Statements | 45-23/24/25-34-39/23/24/25-43-68 |
| Safety Statements | 53-26-36/37/39-45 |
| RIDADR | UN 2209 8 / PGIII |
| WGK Germany | WGK 3 |
| Flash Point(F) | 133.0 °F - closed cup |
| Flash Point(C) | 56.11 °C - closed cup |
| Density | 1.09 g/mL at 20 °C; 1.09 g/mL at 25 °C (lit.) |
| Storage Temp. | room temp |
| UNSPSC | 12352114 |