Formamide
SIGMA/47671 - BioUltra, for molecular biology, ≥99.5% (T)
Synonym: Amide C1; Formic amide
CAS Number: 75-12-7
Empirical Formula (Hill Notation): CH3NO
Molecular Weight: 45.04
EC Number: 200-842-0
MDL Number: MFCD00007941
Linear Formula: HCONH2
Product Type: Chemical
| λ | 10 M in H2O |
| anion traces | chloride (Cl-): ≤1 mg/kg |
| sulfate (SO42-): ≤10 mg/kg | |
| application(s) | genomic analysis |
| assay | ≥99.5% (T) |
| autoignition temp. | 932 °F |
| bp | 210 °C (lit.) |
| cation traces | Ag: ≤5 mg/kg |
| Al: ≤1 mg/kg | |
| As: ≤0.1 mg/kg | |
| Ba: ≤1 mg/kg | |
| Bi: ≤1 mg/kg | |
| Ca: ≤5 mg/kg | |
| Cd: ≤1 mg/kg | |
| Co: ≤1 mg/kg | |
| Cr: ≤1 mg/kg | |
| Cu: ≤1 mg/kg | |
| Fe: ≤1 mg/kg | |
| K: ≤20 mg/kg | |
| Li: ≤1 mg/kg | |
| Mg: ≤1 mg/kg | |
| Mn: ≤1 mg/kg | |
| Mo: ≤1 mg/kg | |
| Na: ≤20 mg/kg | |
| NH4+: ≤1000 mg/kg | |
| Ni: ≤1 mg/kg | |
| Pb: ≤1 mg/kg | |
| Sr: ≤1 mg/kg | |
| Tl: ≤5 mg/kg | |
| Zn: ≤1 mg/kg | |
| density | 1.134 g/mL at 25 °C (lit.) |
| form | liquid |
| grade | for molecular biology |
| ign. residue | ≤0.1% |
| impurities | DNases, none detected |
| insoluble matter, passes filter test | |
| phosphatases, none detected | |
| proteases, none detected | |
| RNases, none detected | |
| InChI | 1S/CH3NO/c2-1-3/h1H,(H2,2 |
| InChI key | ZHNUHDYFZUAESO-UHFFFAOYSA |
| mp | 2-3 °C (lit.) |
| pH | 7-9 (25 °C, 1 M in H2O) |
| product line | BioUltra |
| Quality Level | 200  |
| refractive index | n |
| n |
|
| SMILES string | NC=O |
| solubility | H2O: 10 M at 20 °C, clear, colorless |
| technique(s) | FISH: suitable |
| hybridization: suitable | |
| protein extraction: suitable | |
| UV absorption | λ: 260 nm Amax: ≤0.4 |
| λ: 280 nm Amax: ≤0.1 | |
| vapor density | 1.55 (vs air) |
| vapor pressure | 0.08 mmHg ( 20 °C) |
| 30 mmHg ( 129 °C) |
| Application: | Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures. |
| Application: | Formamide has been used: • as a component in the hybridization buffer for in situ hybridization as a component of solution X to wash the slides to remove the coverslips • in the preparation of denaturation solution for spectral karyotyping (SKY) analysis • in the preparation of 1% formaldehyde solution for the pretreatment of slides for comparative genomic hybridization (CGH) • to prepare pre-hybridization buffer I, II, III, IV, and hybridization buffer I, II, and III for RNA fluorescence in situ hybridization (RNA-FISH) • as an ionizing solvent in the production of formic esters and vitamin, a softener for paper, in animal glues, and water-soluble gums, in molecular biology as a denaturing agent |
| Biochem/physiol Actions: | Formamide serves as a good solvent for polar organic and inorganic compounds with potential catalytic activity in prebiotic processes due to its high dielectric constant. |
| Features and Benefits: | • Versatile and adaptable for a wide variety of laboratory and research applications • BioUltra Grade for your Molecular Biology, Cell Biology and Biochemical research • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications • Free from DNase, RNase, NICKase and proteaseTested for trace levels of Anions and Cations |
| General description: | Formamide, also referred to as Carbamaldehyde, is an amide compound derived from formic acid. It plays a crucial role in biochemical research, particularly in denaturation and renaturation of nucleic acids (RNA and DNA). This includes applications like hybridization, sequencing gel electrophoresis, and electron microscopy. In molecular biology, BioUltra Formamide finds extensive use in various applications such as nucleic acid hybridization buffers, genetic fingerprinting, Southern and Northern blotting, as well as YAC screening. It typically functions by lowering the melting temperature of nucleic acid hybrids, facilitating their manipulation, and can enhance denaturation conditions in sequencing gels for more robust results. |
| Other Notes: | For additional information on our range of Biochemicals , please complete this form . |
| Other Notes: | For the preparation of formamide denaturing polyacrylamide gels; In situ hybridization on human chromosomes |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H351 - H360D - H373 |
| Precautionary statements | P201 - P202 - P260 - P280 - P308 + P313 - P405 |
| Hazard Codes | T |
| Risk Statements | 61-40-48/22 |
| Safety Statements | 53-36/37-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | 305.6 °F |
| Flash Point(C) | 152 °C |
| Purity | ≥99.5% (T) |
| bp | 210 °C (lit.) |
| mp | 2-3 °C (lit.) |
| Density | 1.134 g/mL at 25 °C (lit.) |
| Refractive Index | n |
| UNSPSC | 12352111 |