Fura 2-AM
SIGMA/47989 - BioReagent, suitable for fluorescence, ≥95.0% (HPLC)
Synonym: Fura-2 pentakis(acetoxymethyl) ester
CAS Number: 108964-32-5
Empirical Formula (Hill Notation): C44H47N3O24
Molecular Weight: 1001.85
MDL Number: MFCD00036976
Linear Formula: C44H47N3O24
Product Type: Chemical
assay | ≥95.0% (HPLC) |
fluorescence | λex 355 nm; λem 495 nm in 0.1 M Tris pH 8.0, esterase; 10 mM Ca2+ |
form | solid |
InChI | 1S/C44H47N3O24/c1-25-7-8- |
InChI key | VPSRLGDRGCKUTK-UHFFFAOYSA |
product line | BioReagent |
Quality Level | 100 |
SMILES string | CC(=O)OCOC(=O)CN(CC(=O)OC |
solubility | DMSO: soluble |
storage temp. | −20°C |
suitability | suitable for fluorescence |
Application: | Fura 2-AM is a popular probe used to determine dynamic changes in cytosolic free calcium in intact living cells. |
Features and Benefits: | The major benefits of using the ester form of Fura 2 as Fura 2-AM are: • It is cell-permeable. • It can be loaded into cells easily. Other advantages of using Fura 2-AM over single wavelength dyes is that the ratio signal is independent of : • The dye concentration. • Illumination intensity, and • Optical path length. |
General description: | Fura 2-AM (Fura 2), also known as acetoxy-methyl-ester, is a calcium-specific ratio-metric fluorescent dye. In response to calcium ions, ratio metric dyes like Fura 2 changes their respective excitation or emission spectra. The intracellular calcium concentration is determined by the fluorescence emission ratio or excitation at distinct wavelengths. Fura 2 has an emission peak at 505nm, and the excitation peak ranges between 340-380nm and is the preferred calcium indicator. |
Other Notes: | Membrane-permeable derivative of Fura-2. Used to load the chelator into cells. Hydrolysed enzymatically in the cell to yield Fura-2 which remains within the cell |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥95.0% (HPLC) |
Storage Temp. | −20°C |
UNSPSC | 12352200 |