β-Galactosidase from Escherichia coli
SIGMA/48275 - suitable for enzyme immunoassay, lyophilized, powder, ~140 U/mg
Synonym: β-D-Galactoside galactohydrolase; Lactase
CAS Number: 9031-11-2
EC Number: 232-864-1
MDL Number: MFCD00130623
Product Type: Chemical
| contains | phosphate buffer as stabilizer |
| sucrose as stabilizer | |
| form | powder |
| mol wt | Mr ~135000 |
| quality | lyophilized |
| Quality Level | 100  |
| shipped in | wet ice |
| specific activity | ~140 U/mg |
| storage temp. | −20°C |
| technique(s) | enzyme immunoassay: suitable |
| Application: | Aqueous solution may be used directly for derivatization without previous dialysis or gel permeation chromatography. |
| Biochem/physiol Actions: | β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop. |
| Other Notes: | Used in a highly sensitive enzyme-linked immunoassay of human IgG with 4-methylumbelliferyl-β-D- |
| Packaging: | Bottomless glass bottle. Contents are inside inserted fused cone. |
| Physical properties: | Tetramer molecular weight 465 kDa (subunits 116.3 kDa each) |
| Unit Definition: | 1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol 2-nitrophenyl-β-D-galacto |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ~140 U/mg |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.2.1.23 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |