Glycerol
SIGMA/49767 - BioUltra, for molecular biology, anhydrous, ≥99.5% (GC)
Synonym: 1,2,3-Propanetriol; Glycerin
CAS Number: 56-81-5
Empirical Formula (Hill Notation): C3H8O3
Molecular Weight: 92.09
EC Number: 200-289-5
MDL Number: MFCD00004722
Linear Formula: HOCH2CH(OH)CH2OH
Product Type: Chemical
λ | 5 M in H2O |
anion traces | chloride (Cl-): ≤1 mg/kg |
sulfate (SO42-): ≤10 mg/kg | |
assay | ≥99.5% (GC) |
autoignition temp. | 698 °F |
bp | 182 °C/20 mmHg (lit.) |
cation traces | Ag: ≤5 mg/kg |
Al: ≤1 mg/kg | |
As: ≤0.1 mg/kg | |
Ba: ≤1 mg/kg | |
Bi: ≤1 mg/kg | |
Ca: ≤5 mg/kg | |
Cd: ≤1 mg/kg | |
Co: ≤1 mg/kg | |
Cr: ≤1 mg/kg | |
Cu: ≤1 mg/kg | |
Fe: ≤1 mg/kg | |
K: ≤20 mg/kg | |
Li: ≤1 mg/kg | |
Mg: ≤1 mg/kg | |
Mn: ≤1 mg/kg | |
Mo: ≤1 mg/kg | |
Na: ≤20 mg/kg | |
NH4+: ≤5 mg/kg | |
Ni: ≤1 mg/kg | |
Pb: ≤1 mg/kg | |
Sr: ≤1 mg/kg | |
Tl: ≤5 mg/kg | |
Zn: ≤1 mg/kg | |
density | 1.25 g/mL (lit.) |
form | viscous liquid |
grade | for molecular biology |
ign. residue | ≤0.1% |
impurities | DNases, none detected |
insoluble matter, passes filter test | |
phosphatases, none detected | |
proteases, none detected | |
RNases, none detected | |
InChI | 1S/C3H8O3/c4-1-3(6)2-5/h3 |
InChI key | PEDCQBHIVMGVHV-UHFFFAOYSA |
mp | 20 °C (lit.) |
pH | 5.5-8 (25 °C, 5 M in H2O) |
product line | BioUltra |
Quality Level | 200 |
refractive index | n |
n |
|
SMILES string | OCC(O)CO |
solubility | H2O: 5 M at 20 °C, clear, colorless |
UV absorption | λ: 260 nm Amax: 0.05 |
λ: 280 nm Amax: 0.07 | |
vapor density | 3.1 (vs air) |
vapor pressure | <1 mmHg ( 20 °C) |
Application: | Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel′s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component. |
Biochem/physiol Actions: | Glycerol has been used as • a supplement during cell culture of Mycobacterium tuberculosis and Mycobacterium avium. • a fuel during designing enzymatic biofuel cell. • a liquid composite matrix with 4-HCCA and 3-aminoquinoline for analysis of neutral and acidic glycans. • a matrix for fast atom bombardment MS. • may be employed as liquid matrix for the quantification studies by MALDI (Matrix-assisted laser desorption/ionization mass spectrometry) analysis. |
Biochem/physiol Actions: | Glycerol is hygroscopic in nature and is soluble in water owing to its three hydrophilic alcoholic hydroxyl groups. It can form both inter- and intramolecular hydrogen bonds, making it a very flexible molecule. The physiologic effect of glycerine is due to cell-mediated immunity, increased IgG production and increased histamine release. |
General description: | Glycerol is odourless, colorless, viscous in nature, and exists as a sweet tasting liquid. It can be derived naturally as well as from petrochemical feedstock. Glycerol has a wide variety of applications, and is one of the most valuable and versatile chemical substances in nature. It can be used as an emollient, solvent, sweetening agent, in pharmaceutical formulations, cosmetics, foodstuffs and toiletries. It is very stable and can be easily stored under normal temperature; also it is non-irritating and has no adverse impact to the environment. |
Other Notes: | Component of loading buffer in agarose gel electrophoresis of nucleic acids previously denatured with glyoxal; Preparation of phage and plasmid DNA, for the storage of pure cultures |
Packaging: | 1 L in glass bottle |
Packaging: | 100, 250 mL in glass bottle |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | 390.2 °F - Pensky-Martens c |
Flash Point(C) | 199 °C - Pensky-Martens clo |
Purity | ≥99.5% (GC) |
bp | 182 °C/20 mmHg (lit.) |
mp | 20 °C (lit.) |
Density | 1.25 g/mL (lit.) |
Refractive Index | n |
UNSPSC | 50161901 |