Human Bronchial Epithelial Cells: HBEpC, adult
SIGMA/502-05A
Synonym: HBEpC; HBEpC cells
Product Type: Product-on-demand
Cell Applications : HBEpC culture at low magnification (left) and immunolabeled for cytokeratin 18 (right).
Cell Applications : Characterization of the HBEpC based 3D airway tissue model, showing Millipore Insert, day 14 (A). High resolution confocal imaging (B–G) of the top cell layer (B,D,F) or a cross section (C,E,G). Shown are DAPI-labeled Nuclei (B and C), rhod-phall-labeled Actin (D and E) and merged images (F&G).
| biological source | human bronchi (normal surface epithelium) |
| growth mode | Adherent |
| karyotype | 2n = 46 |
| manufacturer/tradename | Cell Applications, Inc |
| morphology | epithelial |
| packaging | pkg of 500,000 cells |
| Quality Level | 100  |
| relevant disease(s) | asthma; autoimmune diseases; cardiovascular diseases |
| shipped in | dry ice |
| storage temp. | −196°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | epithelial function, tissue repair, cell signaling, lung injury treatment, mechanical and oxidative stress tests, cell differentiation, 3D tissue model, cytokine and growth factor production, apoptosis, cell adhesion molecules, cell permeability, pro-inflammatory signaling, viral fusion mechanisms, gene expression, library screening, mucin secretion |
| Cell Line Origin: | Bronchi |
| Components: | Cell Basal Medium containing 10% FBS & 10% DMSO |
| Disclaimer: | RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization. |
| General description: | Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details. HBEpC provide an excellent model system to study all aspects of epithelial function and disease, particularly those related to airway viral infections, as well as tissue repair mechanisms, signaling changes and potential treatments relevant to lung injuries, mechanical and oxidative stress, inflammation, pulmonary diseases and smoking. When grown on inserts and provided with the liquid/air interface, HBEpC can differentiate into a pseudostriated epithelium and serve as a more physiological 3D tissue model for in vitro studies. |
| Preparation Note: | • 1st passage, >500,000 cells in Cell Basal Medium containing 10% FBS & 10% DMSO • Can be cultured at least 16 doublings |
| Subculture Routine: | Please refer to the HBEpC Culture Protocol . |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −196°C |
| UNSPSC | 41106514 |