Human Tracheal Epithelial Cells: HTEpC, adult
SIGMA/504-05A
Product Type: Product-on-demand
Cell Applications : Top Left: Human Tracheal Epithelial Cells, HTEpC. Top Right: HTEpC immunolabeled with an anti-CK18 antibody. Bottom Left/Right: HTEpC transfected with GFP plasmid DNA.
| biological source | human trachea (normal surface epithelium) |
| growth mode | Adherent |
| karyotype | 2n = 46 |
| manufacturer/tradename | Cell Applications, Inc |
| morphology | epithelial |
| packaging | pkg of 500,000 cells |
| Quality Level | 100  |
| relevant disease(s) | cardiovascular diseases |
| shipped in | dry ice |
| storage temp. | −196°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | epithelial function, tissue repair, cell signaling, lung injury treatment, mechanical and oxidative stress tests, lung lubrication, humidity maintenance, cleaning of respiratory tract, testing of drugs, toxins and carcinogens, respiratory function |
| Cell Line Origin: | Trachea |
| Components: | Basal Medium containing 10% FBS & 10% DMSO |
| Disclaimer: | RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization. |
| General description: | Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details. HTEpC provide a useful model system to study all aspects of epithelial function and disease, particularly those related to airway viral infections, as well as tissue repair mechanisms, signaling changes and potential treatments relevant to lung injuries, mechanical and oxidative stress, pulmonary diseases and smoking. HTEpC have been used in a study investigating the role of goblet cell hyperplasia, possibly mediated by the expression of SPDEF, to the pathogenesis of chronic pulmonary diseases (Park, 2007). Characterization: the cells have a characteristic morphology consistent with an epithelial origin and are positive for epithelial cell marker cytokeratin 18. |
| Preparation Note: | • 1st passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO • Can be cultured at least 16 doublings |
| Subculture Routine: | Please refer to the HTEpC Culture Protocol . |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −196°C |
| UNSPSC | 41106514 |