Human Lung Fibroblasts: HLF, fetal
SIGMA/506-05F
Product Type: Product-on-demand
Cell Applications : Human Lung Fibroblasts, HLF (left). HLF immunolabeled for FSP (right, green).
| biological source | human lung (normal parenchyma) |
| growth mode | Adherent |
| karyotype | 2n = 46 |
| manufacturer/tradename | Cell Applications, Inc |
| morphology | fibroblast |
| packaging | pkg of 500,000 cells |
| Quality Level | 100  |
| relevant disease(s) | asthma; tuberculosis; cardiovascular diseases; cancer |
| shipped in | dry ice |
| storage temp. | −196°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | pulmonary function, gene expression, healing and repair process, differentiation, extracellular matrix production, growth factor and cytokine activation, actin reorganization, synthesis of type II collagen, elastin, and proteoglycans, airway remodeling, pharmaceutical development |
| Cell Line Origin: | Lungs |
| Components: | Basal Medium containing 10% FBS & 10% DMSO |
| Disclaimer: | RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization. |
| General description: | Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details. HLF provide an excellent model system to study many aspects of human pulmonary function and pathophysiology. HLF have been utilized in a number of research publications, for example to: • Demonstrate differential gene expression patterns between fetal and adult lung fibroblasts, in particular showing that fetal fibroblasts express higher level of TGF-β receptor interacting protein 1 (TRIP-1) and consequently are less able to contract collagen than adult lung fibroblasts, leading to better healing of acute injuries in very immature lungs compared to higher levels of fibrosis observed in older patients (Navarro, 2009), and additionally discover that TRIP-1 regulates lung fibroblast/myofibroblast trans-differentiation (Nyp, 2014) • Show that angiotensin II promotes lung fibrosis by activating expression of IL-4, IL-7, and PDGF-D increasing the extracellular matrix formation, and that this process can be blocked by angiotensin receptor inhibitor candesartan (Okada, 2009) • Determine that phosphorylation of C/EBPβ contributes to lung fibrosis (Buck, 2011) • Demonstrate that increased tissue rigidity activates toll-like receptor 2 and NF-κB in lung fibroblasts and induces expression of cytokines, CXCL1-3, IL-8 and TNF-α (Zheng, 2011) • Demonstrate that 2-ethoxyestradiol, a non-estrogenic estradiol metabolite analog, inhibits cell growth and retards the progression of pulmonary fibrosis and hypertension (Tofovic, 2008, 2009) • Identify the hypoxia-induced ROS/RhoA–ROCK dependent actin reorganization as a mediator of myofibroblast transformation and collagen synthesis in lung fibrosis (Ni, 2013) |
| Preparation Note: | • 1st passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO • Can be cultured at least 12 doublings |
| Subculture Routine: | Please refer to the HLF Culture Protocol . |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −196°C |
| UNSPSC | 41106514 |