E-Cadherin human
SIGMA/5085 - recombinant, expressed in E. coli, 0.5 mg protein/mL
Product Type: Chemical
| accession no. | NP_004351.1 |
| assay | ≥90% (SDS-PAGE) |
| biological source | human |
| concentration | 0.5 mg protein/mL |
| description | 0.1 mg recombinant human E-Cadherin in 20 mM Tris-HCl buffer, containing NaCl, KCl, EDTA, L-arginine, DTT and glycerol. |
| form | liquid |
| packaging | pkg of 100 μg |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| sterility | Filtered sterilized solution |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | E-Cadherin has been used as coating matrix protein for 1) maintaining long-term ES or iPS cell culture then combine with proper ES cell culture media, 2) as a coating matrix material for 11R tag recombinant TF intracellular delivery for protein derived iPS protocol with extremely low-level non-specific interaction and 3) as a native antigen for antibody production. Use these recommendations as guidelines to determine the optimal coating conditions for your culture system. 1. Thaw E-Cadherin and dilute to desired concentration using serum-free medium or PBS. The final solution should be sufficiently dilute so that the volume added covers the surface evenly. Note: Use 1 ml PBS per well in a 6-well plate. 2. Add 5 - 10 μg protein to each well and incubate at 2 to 10 °C overnight. 3. After incubation, aspirate remaining material. 4. Plates are ready for use. They may also be stored at 2-8 °C damp or air dried if sterility is maintained. |
| Preparation Note: | Recombinant human E-Cadherin gene (155-710 aa Fragment) was constructed with codon optimization and expressed in non-fusion protein form in E. coli as inclusion bodies. The final product was refolded using a unique "temperature shift inclusion body refolding" technology and chromatographically purified. |
| Sequence: | MDWVIPPISCPENEKGPFPKNLVQI |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |