Guanidine hydrochloride
SIGMA/50933 - BioUltra, for molecular biology, ≥99.5% (AT)
Synonym: Aminoformamidine hydrochloride; Aminomethanamidine hydrochloride; Guanidinium chloride
CAS Number: 50-01-1
Empirical Formula (Hill Notation): CH5N3 · HCl
Molecular Weight: 95.53
EC Number: 200-002-3
MDL Number: MFCD00013026
Linear Formula: NH2C(=NH)NH2 · HCl
Product Type: Chemical
| λ | 6 M in H2O |
| anion traces | sulfate (SO42-): ≤50 mg/kg |
| assay | ≥99.5% (AT) |
| biological source | synthetic |
| cation traces | Al: ≤5 mg/kg |
| As: ≤0.1 mg/kg | |
| Ba: ≤5 mg/kg | |
| Bi: ≤5 mg/kg | |
| Ca: ≤10 mg/kg | |
| Cd: ≤5 mg/kg | |
| Co: ≤5 mg/kg | |
| Cr: ≤5 mg/kg | |
| Cu: ≤5 mg/kg | |
| Fe: ≤5 mg/kg | |
| K: ≤50 mg/kg | |
| Li: ≤5 mg/kg | |
| Mg: ≤5 mg/kg | |
| Mn: ≤5 mg/kg | |
| Mo: ≤5 mg/kg | |
| Na: ≤150 mg/kg | |
| Ni: ≤5 mg/kg | |
| Pb: ≤5 mg/kg | |
| Sr: ≤5 mg/kg | |
| Zn: ≤5 mg/kg | |
| color | colorless to white, |
| density | 1.3 g/cm3 (lit.) |
| form | powder or crystals |
| grade | for molecular biology |
| ign. residue | ≤0.05% (as SO4) |
| impurities | DNases, none detected |
| insoluble matter, passes filter test | |
| phosphatases, none detected | |
| proteases, none detected | |
| RNases, none detected | |
| InChI | 1S/CH5N3.ClH/c2-1(3)4;/h( |
| InChI key | PJJJBBJSCAKJQF-UHFFFAOYSA |
| loss | ≤0.05% loss on drying, 20 °C (HV) |
| mp | 180-185 °C (lit.) |
| pH | 4.5-6.0 (25 °C, 6 M in H2O) |
| product line | BioUltra |
| Quality Level | 200  |
| SMILES string | Cl[H].NC(N)=N |
| solubility | H2O: 6 M at 20 °C, clear, colorless |
| storage temp. | room temp |
| technique(s) | RNA extraction: suitable |
| UV absorption | λ: 260 nm Amax: 0.03 |
| λ: 280 nm Amax: 0.02 |
| Application: | Guanidine hydrochloride (GdnHCl) has been used as a component of the phosphate buffer saline (PBS) for the incubation of Schlemm’s canal (SC) cells. It has also been used as a component of the digestion buffer for the gelation and expansion of human HeLa229 cells. GdnHCl is also used to isolate RNA. |
| Application: | Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. |
| Biochem/physiol Actions: | Guanidine hydrochloride (GdnHCl) is involved in conformational changes and loss of enzyme activity due to inactivation of the enzyme. It is involved in the loss of activity of alkaline phosphatase (ALPase) enzyme in Haliotis diversicolor. GdnHCl functions by hampering the activity of heat shock protein 104 (Hsp104) adenosine triphosphatase (ATPase) in vivo. It can also inactivate enzymes like papain and aminocyclase. Higher concentrations of GdnHCl leads to viral inactivation of herpes simplex virus 1 (HSV-1). |
| General description: | Guanidine hydrochloride (GdnHCl) is a well-known protein and enzyme denaturant. It is a small molecule and hydroscopic in nature. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H302 + H332 - H315 - H319 |
| Precautionary statements | P261 - P264 - P301 + P312 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 22-36/38 |
| Safety Statements | 22 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99.5% (AT) |
| mp | 180-185 °C (lit.) |
| Density | 1.3 g/cm3 (lit.) |
| Storage Temp. | room temp |
| UNSPSC | 12352107 |