Guanidine hydrochloride solution
SIGMA/50937 - BioUltra, ~8 M in H2O
Synonym: Aminoformamidine hydrochloride; Guanidinium chloride; Guanidium chloride
CAS Number: 50-01-1
Empirical Formula (Hill Notation): CH5N3 · HCl
Molecular Weight: 95.53
MDL Number: MFCD00013026
Linear Formula: CH5N3 · HCl
Product Type: Chemical
λ | neat |
anion traces | sulfate (SO42-): ≤50 mg/kg |
cation traces | Al: ≤1 mg/kg |
As: ≤0.1 mg/kg | |
Ba: ≤1 mg/kg | |
Bi: ≤1 mg/kg | |
Ca: ≤5 mg/kg | |
Cd: ≤1 mg/kg | |
Co: ≤1 mg/kg | |
Cr: ≤1 mg/kg | |
Cu: ≤1 mg/kg | |
Fe: ≤1 mg/kg | |
K: ≤20 mg/kg | |
Li: ≤1 mg/kg | |
Mg: ≤1 mg/kg | |
Mn: ≤1 mg/kg | |
Mo: ≤1 mg/kg | |
Na: ≤60 mg/kg | |
Ni: ≤1 mg/kg | |
Pb: ≤1 mg/kg | |
Sr: ≤1 mg/kg | |
Zn: ≤1 mg/kg | |
concentration | ~8 M in H2O |
form | liquid |
impurities | insoluble matter, passes filter test |
InChI | 1S/CH5N3.ClH/c2-1(3)4;/h( |
InChI key | PJJJBBJSCAKJQF-UHFFFAOYSA |
pH | 4.5-7.5 (25 °C) |
product line | BioUltra |
Quality Level | 100 |
refractive index | n |
SMILES string | Cl.NC(N)=N |
UV absorption | λ: 260 nm Amax: 0.040 |
λ: 280 nm Amax: 0.025 |
Application: | Guanidine hydrochloride solution has been used as a fixation buffer in genomic DNA extraction for genomic analysis. |
Biochem/physiol Actions: | Guanidine‐HCl (Gnd-HCl), an efficient chaotropic agent, is broadly used in RNA isolation, globular protein denaturation, and protein refolding studies as a denaturant. It is an appropriate sample buffer for the quick and effective elution of affinity-purified protein complexes and the subsequent protein digestion step. Gnd-HCl is not preferred in proteomics experiments due to its hindrance with trypsin activity at low molar concentrations and tendency to precipitate when combined with sodium dodecyl sulfate (SDS). GnHCl is commonly utilized to extract proteins in extracellular matrix (ECM)‐rich tissues including bone, cartilage, ligament, and tendon. |
Symbol | GHS07 |
Signal word | Warning |
Hazard statements | H302 + H332 - H315 - H319 |
Precautionary statements | P261 - P264 - P301 + P312 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 |
Hazard Codes | Xn |
Risk Statements | 22-36/38 |
Safety Statements | 26 |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Refractive Index | n |
UNSPSC | 12352107 |