Guanidine thiocyanate
SIGMA/50980 - BioUltra, for molecular biology, ≥99.0% (AT)
Synonym: Guanidinium rhodanide; Guanidinium thiocyanate
CAS Number: 593-84-0
Empirical Formula (Hill Notation): CH5N3 · CHNS
Molecular Weight: 118.16
EC Number: 209-812-1
MDL Number: MFCD00013027
Linear Formula: NH2C(=NH)NH2 · HSCN
Product Type: Chemical
| absorption | cut-off at 280 nm in H2O at 6 M |
| anion traces | chloride (Cl-): ≤5000 mg/kg |
| sulfate (SO42-): ≤50 mg/kg | |
| assay | ≥99.0% (AT) |
| cation traces | Al: ≤5 mg/kg |
| As: ≤0.1 mg/kg | |
| Ba: ≤5 mg/kg | |
| Bi: ≤5 mg/kg | |
| Ca: ≤10 mg/kg | |
| Cd: ≤5 mg/kg | |
| Co: ≤5 mg/kg | |
| Cr: ≤5 mg/kg | |
| Cu: ≤5 mg/kg | |
| Fe: ≤5 mg/kg | |
| K: ≤50 mg/kg | |
| Li: ≤5 mg/kg | |
| Mg: ≤5 mg/kg | |
| Mn: ≤5 mg/kg | |
| Mo: ≤5 mg/kg | |
| Na: ≤5000 mg/kg | |
| Ni: ≤5 mg/kg | |
| Pb: ≤5 mg/kg | |
| Sr: ≤5 mg/kg | |
| Zn: ≤5 mg/kg | |
| form | solid |
| grade | for molecular biology |
| ign. residue | ≤0.5% (as SO4) |
| impurities | DNases, none detected |
| insoluble matter, passes filter test | |
| phosphatases, none detected | |
| proteases, none detected | |
| RNases, none detected | |
| InChI | 1S/CH5N3.CHNS/c2-1(3)4;2- |
| InChI key | ZJYYHGLJYGJLLN-UHFFFAOYSA |
| loss | ≤0.1% loss on drying, 20 °C (HV) |
| mp | 117-120 °C |
| 120-122 °C (lit.) | |
| pH | 4.5-7.0 (25 °C, 6 M in H2O) |
| product line | BioUltra |
| Quality Level | 100  |
| SMILES string | SC#N.NC(N)=N |
| solubility | H2O: 6 M at 20 °C, clear, colorless |
| Application: | Chaotropic agent and strong denaturant; solubilizes cells. |
| Other Notes: | Isolation of RNA; A new guanidine thiocyanate procedure for the isolation of full-length RNA templates for reverse transcription from tissues rich in RNase and proteoglycans |
| Symbol |   GHS05,GHS07 |
| Signal word | Danger |
| Hazard statements | H302 + H312 + H332 - H314 - H412 |
| Precautionary statements | P260 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P312 - P305 + P351 + P338 + P310 |
| Hazard Codes | C,Xn |
| Risk Statements | 20/21/22-32-34-52/53 |
| Safety Statements | 13-26-36/37/39-45-61 |
| RIDADR | UN 1759 8 / PGIII |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Supplemental Hazard Statements | EUH032 |
| Purity | ≥99.0% (AT) |
| mp | 117-120 °C; 120-122 °C (lit.) |
| UNSPSC | 12352107 |