Guanidine thiocyanate
SIGMA/50981 - BioReagent, for molecular biology, ≥99%
Synonym: Guanidinium rhodanide; Guanidinium thiocyanate
CAS Number: 593-84-0
Empirical Formula (Hill Notation): CH5N3 · CHNS
Molecular Weight: 118.16
EC Number: 209-812-1
MDL Number: MFCD00013027
Linear Formula: NH2C(=NH)NH2 · HSCN
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: 50981-100G
This image depicts SKU: 50981-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 50981-500G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: 50981-500G
This image depicts SKU: 50981-500G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 50981-100G
| anion traces | chloride (Cl-): ≤5000 mg/kg |
| assay | ≥99% |
| biological source | synthetic |
| cation traces | Al: ≤5 mg/kg |
| As: ≤0.1 mg/kg | |
| Ba: ≤5 mg/kg | |
| Bi: ≤5 mg/kg | |
| Ca: ≤10 mg/kg | |
| Cd: ≤5 mg/kg | |
| Co: ≤5 mg/kg | |
| Cr: ≤5 mg/kg | |
| Cu: ≤5 mg/kg | |
| Fe: ≤5 mg/kg | |
| K: ≤50 mg/kg | |
| Li: ≤5 mg/kg | |
| Mg: ≤5 mg/kg | |
| Mn: ≤5 mg/kg | |
| Mo: ≤5 mg/kg | |
| Na: ≤5000 mg/kg | |
| Ni: ≤5 mg/kg | |
| Pb: ≤5 mg/kg | |
| Sr: ≤5 mg/kg | |
| Zn: ≤5 mg/kg | |
| color | colorless to white, |
| form | powder |
| grade | for molecular biology |
| impurities | DNases, none detected |
| phosphatases, none detected | |
| proteases, none detected | |
| RNases, none detected | |
| InChI | 1S/CH5N3.CHNS/c2-1(3)4;2- |
| InChI key | ZJYYHGLJYGJLLN-UHFFFAOYSA |
| mp | 115-122 °C |
| 120-122 °C (lit.) | |
| product line | BioReagent |
| Quality Level | 200  |
| SMILES string | SC#N.NC(N)=N |
| solubility | H2O: 4 M at 20 °C, clear, colorless |
| storage temp. | room temp |
| technique(s) | RNA extraction: suitable |
| Application: | Chaotropic agent and strong denaturant; solubilizes cells. |
| Application: | Guanidine thiocyanate has been used as a component of the stabilization buffer for RNA lysis. It can be used for deactivation of endogenous RNases leading to denaturation of nucleoprotein complexes. It is also suitable for denaturation of macromolecules such as nucleic acids and proteins and for RNA isolation. |
| Biochem/physiol Actions: | Guanidine thiocyanate (GTC) plays a role in protein denaturation. It is involved in separation of ribosomal ribonucleic acid (rRNA) from ribosomes and denatures ribonuclease (RNase). GTC has been studied in the unfolding and refolding of fluorescent proteins (FP). It can be used along with phenol and chloroform for extraction of RNA. |
| Packaging: | 100, 500 g in glass bottle |
| Principle: | Guanidine thiocyanate interferes with the hydrogen bonds and intramolecular interactions of macromolecules. It solubilizes the proteins resulting in disintegration of cellular structures. |
| Symbol |   GHS05,GHS07 |
| Signal word | Danger |
| Hazard statements | H302 + H312 + H332 - H314 - H412 |
| Precautionary statements | P260 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 |
| Hazard Codes | C,Xn |
| Risk Statements | 20/21/22-32-34-52/53 |
| Safety Statements | 13-26-36/37/39-45-61 |
| RIDADR | UN 1759 8 / PGIII |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Supplemental Hazard Statements | EUH032 |
| Purity | ≥99% |
| mp | 115-122 °C; 120-122 °C (lit.) |
| Storage Temp. | room temp |
| UNSPSC | 12352107 |