Guanidine thiocyanate solution
SIGMA/50983 - BioUltra, for molecular biology, ~6 M in H2O
Synonym: Guanidine isothiocyanate; Guanidinium isothiocyanate; Guanidinium rhodanide; Guanidinium thiocyanate
CAS Number: 593-84-0
Empirical Formula (Hill Notation): CH5N3 · CHNS
Molecular Weight: 118.16
MDL Number: MFCD00013027
Linear Formula: NH2C(=NH)NH2 · HSCN
Product Type: Chemical
absorption | cut-off at 280 nm |
anion traces | chloride (Cl-): ≤500 mg/kg |
sulfate (SO42-): ≤50 mg/kg | |
cation traces | Al: ≤1 mg/kg |
As: ≤0.1 mg/kg | |
Ba: ≤1 mg/kg | |
Bi: ≤1 mg/kg | |
Ca: ≤5 mg/kg | |
Cd: ≤1 mg/kg | |
Co: ≤1 mg/kg | |
Cr: ≤1 mg/kg | |
Cu: ≤1 mg/kg | |
Fe: ≤1 mg/kg | |
K: ≤20 mg/kg | |
Li: ≤1 mg/kg | |
Mg: ≤1 mg/kg | |
Mn: ≤1 mg/kg | |
Mo: ≤1 mg/kg | |
Na: ≤500 mg/kg | |
Ni: ≤1 mg/kg | |
Pb: ≤1 mg/kg | |
Sr: ≤1 mg/kg | |
Zn: ≤1 mg/kg | |
concentration | ~6 M in H2O |
grade | for molecular biology |
impurities | DNases, none detected |
insoluble matter, passes filter test | |
phosphatases, none detected | |
proteases, none detected | |
RNases, none detected | |
InChI | 1S/CH5N3.CHNS/c2-1(3)4;2- |
InChI key | ZJYYHGLJYGJLLN-UHFFFAOYSA |
pH | 4.5-7.0 (25 °C) |
product line | BioUltra |
Quality Level | 200 |
refractive index | n |
SMILES string | SC#N.NC(N)=N |
Application: | Guanidine thiocyanate solution has been used to extract: • plasma RNA from human immunodeficiency virus (HIV)-infected and control patients • total RNA from collected tissues (shoots, flowers, fruits, and seeds) • in reverse transcription-polymerase chain reaction (RT-PCR) analysis, RNA from teratocarcinoma cell line (Tera-1) |
Caution: | possibly crystallized product can be dissolved again by slightly heating to 40-50 °C. |
General description: | Guanidine thiocyanate is a chaotropic agent that functions as a strong protein denaturant. It is widely used in RNA isolation protocols. Guanidine thiocyanate can irreversibly inactivate RNase and DNase and breaks cellular membranes. It dissolves proteins by disturbing their secondary structures. Guanidine thiocyanate also separates nucleoproteins from nucleic acids and aids the digestion of the proteins with proteases. It improves the activity of proteinase K thus making it the protease of choice. |
Symbol | GHS05,GHS07 |
Signal word | Danger |
Hazard statements | H302 + H312 + H332 - H314 - H412 |
Precautionary statements | P273 - P280 - P301 + P312 + P330 - P303 + P361 + P353 - P304 + P340 + P312 - P305 + P351 + P338 + P310 |
Hazard Codes | C |
Risk Statements | 20/21/22-32-34-52/53 |
Safety Statements | 7-26-36/37/39-45-60 |
RIDADR | UN 3267 8 / PGIII |
WGK Germany | WGK 2 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Supplemental Hazard Statements | EUH032 |
Refractive Index | n |
UNSPSC | 12352107 |