TRIS borate-EDTA buffer concentrate, long run
SIGMA/51309 - for Northern and Southern blotting, solution
MDL Number: MFCD00217350
Product Type: Chemical
form | solution |
grade | for molecular biology |
impurities | DNases, none detected |
phosphatases, none detected | |
proteases, none detected | |
RNases, none detected | |
product line | BioReagent |
quality | 10× concentrate |
ready-to-use solution | |
Quality Level | 100 |
storage temp. | room temp |
Application: | Suitable for use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures. |
Components: | The TBE 10x concentrate contains 1.3M TRIS, 450 mM boric acid and 25 mM EDTA in water. |
General description: | TBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution is effective under slightly basic conditions, which keeps DNA deprotonated , water-soluble, and protected from degradation. This concentrate can be easily diluted to 1x or 0.5x before use (with molecular biology grade water). |
Symbol | GHS08 |
Signal word | Danger |
Hazard statements | H360FD |
Precautionary statements | P201 - P202 - P280 - P308 + P313 - P405 - P501 |
Hazard Codes | T |
Risk Statements | 60-61 |
Safety Statements | 53-45 |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Storage Temp. | room temp |
UNSPSC | 12161700 |