Minimum Essential Medium
SIGMA/51412C - with Earle′s Balanced Salts, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
Synonym: Minimum Essential Medium Eagle; MEM
Product Type: Chemical
| components | L-glutamine: no NaHCO3: 2200 mg/L phenol red: 10.62 mg/L Earle’s salts (5% CO2): yes |
| description | for research or for further manufacturing use |
| form | liquid |
| Quality Level | 500  |
| sterility | sterile-filtered |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| cell culture | stem cell: suitable |
| Application: | Minimum essential medium (MEM) has been used as a growth medium for bone marrow mesenchymal stem cells (BMSCs) and neurons. |
| General description: | Minimum Essential Medium (MEM) with Earle’s Balanced Salts was developed by Harry Eagle as a result of his studies to determine the essential nutritional requirements of mouse L cells and HeLa cells in culture. He demonstrated that these cells require 13 amino acids and seven vitamins to grow and reproduce in vitro. MEM is a modification of Eagle’s earlier medium Basal Medium Eagle (BME), containing higher concentrations of the essential nutrients. This medium has demonstrated the ability to support a variety of normal and transformed cells in culture and contains Earle’s Balanced Salts, which makes it suitable for use in atmospheres charged with CO2 gas. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |