Urea
SIGMA/51456 - BioUltra, Molecular Biology, 99% (T)
Synonym: Carbamide; Carbonyldiamide
CAS Number: 57-13-6
Empirical Formula (Hill Notation): CH4N2O
Molecular Weight: 60.06
EC Number: 200-315-5
MDL Number: MFCD00008022
Linear Formula: NH2CONH2
Product Type: Chemical
| λ | 8 M in H2O |
| anion traces | chloride (Cl-): ≤5 mg/kg |
| sulfate (SO42-): ≤10 mg/kg | |
| assay | 99% (T) |
| cation traces | Al: ≤5 mg/kg |
| As: ≤0.1 mg/kg | |
| Ba: ≤5 mg/kg | |
| Bi: ≤5 mg/kg | |
| Ca: ≤10 mg/kg | |
| Cd: ≤5 mg/kg | |
| Co: ≤5 mg/kg | |
| Cr: ≤5 mg/kg | |
| Cu: ≤5 mg/kg | |
| Fe: ≤5 mg/kg | |
| K: ≤50 mg/kg | |
| Li: ≤5 mg/kg | |
| Mg: ≤5 mg/kg | |
| Mn: ≤5 mg/kg | |
| Mo: ≤5 mg/kg | |
| Na: ≤50 mg/kg | |
| Ni: ≤5 mg/kg | |
| Pb: ≤5 mg/kg | |
| Sr: ≤5 mg/kg | |
| Zn: ≤5 mg/kg | |
| density | 1.335 g/mL at 25 °C (lit.) |
| form | powder or crystals |
| grade | Molecular Biology |
| ign. residue | ≤0.01% (as SO4) |
| impurities | DNases, none detected |
| insoluble matter, passes filter test | |
| phosphatases, none detected | |
| proteases, none detected | |
| RNases, none detected | |
| ≤0.1% biuret | |
| <0.005% formaldehyde (HCHO) | |
| InChI | 1S/CH4N2O/c2-1(3)4/h(H4,2 |
| InChI key | XSQUKJJJFZCRTK-UHFFFAOYSA |
| loss | ≤0.1% loss on drying, 20 °C (HV) |
| mp | 132-135 °C (lit.) |
| pH | 8.0-10.0 (20 °C, 8 M in H2O) |
| product line | BioUltra |
| Quality Level | 200  |
| SMILES string | NC(N)=O |
| solubility | H2O: 8 M at 20 °C, clear, colorless |
| UV absorption | λ: 260 nm Amax: ≤0.03 |
| λ: 280 nm Amax: ≤0.02 |
| Application: | Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. |
| Other Notes: | Denaturing reagent; Commonly used in conjunction with polyacrylamide gel electrophoresis for the fractionation of single-stranded nucleic acids with chains of up to 200 nucleotides |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | 99% (T) |
| mp | 132-135 °C (lit.) |
| Density | 1.335 g/mL at 25 °C (lit.) |
| UNSPSC | 12352111 |