Atto MB2 maleimide
SIGMA/51797 - Methylene Blue derivative, ≥90% (HPLC)
Product Type: Chemical
| λ | in ethanol (with 0.1% trifluoroacetic acid) |
| assay | ≥90% (degree of coupling) |
| ≥90% (HPLC) | |
| form | solid |
| manufacturer/tradename | ATTO-TEC GmbH |
| Quality Level | 100  |
| storage temp. | −20°C |
| UV absorption | λ: 655-661 nm Amax |
| Application: | ATTO MB2 is a derivative of the well-known redox dye Methylene Blue. The dye can be reversibly reduced to the colorless leuko form. Upon oxidation the dye recovers and the absorption is fully restored. In common with most ATTO-labels the dye shows a high extinction coefficient. The dye is moderately hydrophilic. ATTO MB2 is a cationic dye. After coupling to a substrate the dye carries a net electrical charge of +1. Maleimides are well suited for coupling to thiol groups. This is similar to iodacetamides, but maleimides react more selectively with thiols. They do not show significant reaction with histidine or methionine. Hydrolysis of maleimides to a mixture of isomeric nonreactive maleamic acids can compete significantly with thiol modification, particularly above pH 8. Maleimides may be used for labeling of amines, which usually requires a higher pH than reaction of maleimides with thiols. |
| Legal Information: | This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (HPLC); ≥90% (degree of coupling) |
| Storage Temp. | −20°C |
| UNSPSC | 12352111 |