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Claycomb Medium

SIGMA/51800C - without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym: HL-1 Medium

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-51800C-500ML 500 mL
$206.00
1/EA
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components L-glutamine: no
description For research or for further manufacturing use.
form liquid
Quality Level 500 
shipped in ambient
sterility sterile-filtered
storage condition protect from light
storage temp. 2-8°C
technique(s) cell culture | mammalian: suitable
Application: Claycomb Medium, named after Dr. William Claycomb who established the HL-1 cell line, is specifically designed for the growth of murine cardiomyocytes. HL-1 is the first cell line established that can maintain the differentiated cardiomyocyte phenotype and contractile activity in vitro. The HL-1 cell line can be used for the study of cardiac cell hypertrophy that follows myocardial infarction, the testing of novel cardiac therapeutic drugs and treatments, the production of high levels of cardiac proteins and the study of mature cardiomyocyte specific genes. Claycomb Medium, when supplemented with 100 μM norepinephrin, 10% fetal bovine serum (FBS) and 4 mM L-glutamine, will maintain the HL-1 cell line and the mature cardiomycyte behavior. While observing the HL-1 cells under light microscopy, individual and groups of cells can be observed contracting, becoming more frequent as the cardiomyocytes reach confluency.
RIDADR NONH for all modes of transport
WGK Germany WGK 1
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. 2-8°C
UNSPSC 12352207

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