Lipase B Candida antarctica immobilized on Immobead 150, recombinant from yeast
SIGMA/52583 - ≥2000 U/g
Product Type: Chemical
form | beads |
mol wt | 33 kDa |
Quality Level | 100 |
recombinant | expressed in yeast |
specific activity | ≥2000 U/g |
storage temp. | 2-8°C |
Application: | Lipase B Candida antarctica immobilized on Immobead 150, recombinant from yeast has been used: • in esterification reaction of lauric acid with n-butanol in a biphasic solvent system • in hydrolysis of fish oil triglycerides • in screening of enzymes for Morita–Baylis–Hillman (MBH) reaction |
Application: | Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel. |
Biochem/physiol Actions: | Lipase B from Candida antarctica has been shown to be an effective catalyst for the synthesis of esters of ethyl D-glucopyranoside from fatty acids larger than octanoic acid. It has also been found to catalyze a wide variety of organic reactions including many different regio- and enantio-selective syntheses. |
Biochem/physiol Actions: | Lipases B from Candida antarctica (CALB) is a versatile catalyst for biotransformation reactions. In aqueous media, CALB behaves like an esterase rather than a lipase. |
Biochem/physiol Actions: | Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids. |
General description: | Lipase B or triacylglycerol ester hydrolases belongs to the class of hydrolases. Lipase B from Candida antarctica (CALB) possesses 317 amino acids and a molecular weight of 33 kDa. CALB has a catalytic triad and an open solvent accessible active site. The catalytic triad is usually Ser-His-Asp/Glu which is found in the carboxy terminal of parallel β sheet. |
Unit Definition: | 1 U corresponds to the amount of enzyme which liberates 1 μmol butyric acid per minute at pH 7.5 and 40°C (tributyrin, Cat. No. 91010, as substrate) |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
activity | specific activity: ≥2000 U/g |
Storage Temp. | 2-8°C |
UNSPSC | 12352204 |