HEPES
SIGMA/54457 - BioUltra, for molecular biology, ≥99.5% (T)
Synonym: 4-
CAS Number: 7365-45-9
Empirical Formula (Hill Notation): C8H18N2O4S
Molecular Weight: 238.30
EC Number: 230-907-9
MDL Number: MFCD00006158
Linear Formula: C8H18N2O4S
Product Type: Chemical
| λ | 1 M in H2O |
| anion traces | chloride (Cl-): ≤50 mg/kg |
| sulfate (SO42-): ≤50 mg/kg | |
| assay | ≥99.5% (T) |
| cation traces | Al: ≤5 mg/kg |
| As: ≤0.1 mg/kg | |
| Ba: ≤5 mg/kg | |
| Bi: ≤5 mg/kg | |
| Ca: ≤10 mg/kg | |
| Cd: ≤5 mg/kg | |
| Co: ≤5 mg/kg | |
| Cr: ≤5 mg/kg | |
| Cu: ≤5 mg/kg | |
| Fe: ≤5 mg/kg | |
| K: ≤50 mg/kg | |
| Li: ≤5 mg/kg | |
| Mg: ≤5 mg/kg | |
| Mn: ≤5 mg/kg | |
| Mo: ≤5 mg/kg | |
| Na: ≤50 mg/kg | |
| Ni: ≤5 mg/kg | |
| Pb: ≤5 mg/kg | |
| Sr: ≤5 mg/kg | |
| Zn: ≤5 mg/kg | |
| form | powder |
| grade | for molecular biology |
| ign. residue | ≤0.1% (as SO4) |
| impurities | DNases, none detected |
| insoluble matter, passes filter test | |
| phosphatases, none detected | |
| proteases, none detected | |
| RNases, none detected | |
| InChI | 1S/C8H18N2O4S/c11-7-5-9-1 |
| InChI key | JKMHFZQWWAIEOD-UHFFFAOYSA |
| loss | ≤0.5% loss on drying, 110 °C |
| pH | 5.0-6.5 (25 °C, 1 M in H2O) |
| pKa (25 °C) | 7.5 |
| product line | BioUltra |
| Quality Level | 200  |
| SMILES string | OCCN1CCN(CC1)CCS(O)(=O)=O |
| solubility | H2O: 1 M at 20 °C, clear, colorless |
| useful pH range | 6.8-8.2 |
| UV absorption | λ: 260 nm Amax: 0.045 |
| λ: 280 nm Amax: 0.035 |
| Application: | HEPES has been used as a component of: • DMEM (Dulbecco′s modified Eagle′s medium) used for the cultivation of CHO-GPR40?cells • RNA buffer (for gel-filtration chromatography) and transcription buffer (for transcription reaction) • Recapture solution to re-capture RNA exosomes from the glycerol gradient • ExoI and ExoII extraction solution as well as light and heavy gradient solution for gradient centrifugation • 2X HBS buffer that is used in transfection process for the production of recombinant lentivectors |
| General description: | HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg. |
| Other Notes: | 3′-Terminal labeling of RNA with T4 RNA ligase; Isolation and analysis of nuclear RNA - component of reaction buffer, prehybridization buffer, hybridization buffer. |
| Other Notes: | Easily compare specifications for HEPES products with the HEPES specification table . |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99.5% (T) |
| UNSPSC | 12161700 |