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Digoxigenin NHS-ester

SIGMA/55865 - ≥80% (HPLC)

Synonym: ε-(Digoxigenin-3-0-acetamido)caproic acid N-hydroxysuccinimide ester; DIG NHS

CAS Number: 129273-26-3
Empirical Formula (Hill Notation): C35H50N2O10
Molecular Weight: 658.78
MDL Number: MFCD02093394
Linear Formula: C35H50N2O10
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-55865-1MG-F 1 mg
 $326.00
1/EA		 Add To Favorites
45-55865-5MG-F 5 mg
 $1150.00
1/EA		 Add To Favorites

 

assay ≥80% (HPLC)
form solid
InChI 1S/C35H50N2O10/c1-33-13-11-23(45-20-28(39)36-15-5-3-4-6-31(42)47-37-29(40)9-10-30(37)41)17-22(33)7-8-25-26(33)18-27(38)34(2)24(12-14-35(25,34)44)21-16-32(43)46-19-21/h16,22-27,38,44H,3-15,17-20H2,1-2H3,(H,36,39)/t22-,23+,24-,25-,26+,27-,33+,34+,35+/m1/s1
InChI key KHNDABJZSPPYLE-FUGFVFQCSA-N
Quality Level 200 
shipped in wet ice
SMILES string [H][C@]12CC[C@]3([H])[C@]([H])(C[C@@H](O)[C@]4(C)[C@H](CC[C@]34O)C5=CC(=O)OC5)[C@@]1(C)CC[C@@H](C2)OCC(=O)NCCCCCC(=O)ON6C(=O)CCC6=O
storage temp. −20°C
Application: Digoxigenin NHS-ester has been used in the preparation of streptavidin-digoxigenin conjugate and digoxin-recombinant Ara h1 (peanut component) conjugate for enzyme-linked immunosorbent assay (ELISA) analysis.
Biochem/physiol Actions: Digoxigenin NHS-ester is an activated ester that reacts with amino groups under mild conditions, attaching the digoxigenin moiety to proteins or 5′-amino substituted oligonucleotides Digoxigenin labeling is used to immuno-tag molecules for detection using anti-digoxigenin antibodies with high affinities and specificity.
Biochem/physiol Actions: Digoxigenin, an aglycon, is a pharmacologically active plant toxin. It is less potent than digoxin. It is used in lower doses to treat heart conditions. Digoxigenin is usually conjugated to another payload which reduces its remaining activity or accessibility to tissues. Since digoxigenin is permanently bound and entirely covered by the capture modules, it cannot be used as an active entity.
Purity ≥80% (HPLC)
Storage Temp. −20°C
UNSPSC 12352108

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