Synonym: Calcein O,O′-diacetate tetrakis(acetoxymethyl) ester; fluorescein complexone; Calcein O,O′-diacetate tetrakis(acetoxymethyl) ester
CAS Number: 148504-34-1
Empirical Formula (Hill Notation): C46H46N2O23
Molecular Weight: 994.86
MDL Number: MFCD05861516
Linear Formula: C46H46N2O23
Product Type: Chemical
| assay |
≥95.0% (HPLC) |
| fluorescence |
λex 496 nm; λem 516 nm±5 nm in 0.1 M Tris pH 8.0, esterase; Ca2+ |
| InChI |
1S/C46H46N2O23/c1-25(49)60-21-64-39(55)17-47(18-40(56)65-22-61-26(2)50)15-32-37(68-29(5)53)13-11-35-43(32)70-44-33(16-48(19-41(57)66-23-62-27(3)51)20-42(58)67-24-63-28(4)52)38(69-30(6)54)14-12-36(44)46(35)34-10-8-7-9-31(34)45(59)71-46/h7-14H,15-24H2,1-6H3 |
| InChI key |
XKFSBWQWNMZWFA-UHFFFAOYSA-N |
| Quality Level |
100  |
| SMILES string |
CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)Cc1c(OC(C)=O)ccc2c1Oc3c(CN(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)c(OC(C)=O)ccc3C24OC(=O)c5ccccc45 |
| storage temp. |
−20°C |
| Application: |
Calcein-AM is used as a cell viability stain and as a neutral substrate for multidrug (MDR) efflux transporters. In cancer research, it is used as a neutral substrate for multidrug resistance protein MRP. Fluorescence assays to determine human erythrocyte viability and aging uses Calcein-AM as a probe. |
| Application: |
Non-fluorescent cell permeable derivative of calcein, becomes fluorescent on hydrolysis. Used as a neutral substrate for multidrug resistance protein, MRP; employed in tumor research. |
| General description: |
Calcein-AM, also known as Calcein-acetoxymethyl ester, is a non-fluorescent molecule converted into an anionic fluorescent form by intracellular esterase enzymes. It provides a continuous visualization of adherent cells during the experiment. Calcein-AM is a vital dye that introduces Calcein in living cells with intact cell membranes. The fluorescent part of Calcein-AM localizes in the intracellular spaces after esterase-dependent cellular trapping, displaying cytotoxic activity in cells. |
| RIDADR |
NONH for all modes of transport |
| WGK Germany |
WGK 3 |
| Flash Point(F) |
Not applicable |
| Flash Point(C) |
Not applicable |
| Purity |
≥95.0% (HPLC) |
| Storage Temp. |
−20°C |
| UNSPSC |
12352200 |
