Imidazole
SIGMA/56749 - BioUltra, ≥99.5% (GC)
Synonym: 1,3-
CAS Number: 288-32-4
Empirical Formula (Hill Notation): C3H4N2
Molecular Weight: 68.08
EC Number: 206-019-2
MDL Number: MFCD00005183
Linear Formula: C3H4N2
Product Type: Chemical
| λ | 0.1 M in H2O |
| anion traces | chloride (Cl-): ≤50 mg/kg |
| sulfate (SO42-): ≤50 mg/kg | |
| assay | ≥99.5% (GC) |
| bp | 256 °C (lit.) |
| cation traces | Al: ≤5 mg/kg |
| As: ≤0.1 mg/kg | |
| Ba: ≤5 mg/kg | |
| Bi: ≤5 mg/kg | |
| Ca: ≤10 mg/kg | |
| Cd: ≤5 mg/kg | |
| Co: ≤5 mg/kg | |
| Cr: ≤5 mg/kg | |
| Cu: ≤5 mg/kg | |
| Fe: ≤5 mg/kg | |
| K: ≤50 mg/kg | |
| Li: ≤5 mg/kg | |
| Mg: ≤5 mg/kg | |
| Mn: ≤5 mg/kg | |
| Mo: ≤5 mg/kg | |
| Na: ≤50 mg/kg | |
| Ni: ≤5 mg/kg | |
| Pb: ≤5 mg/kg | |
| Sr: ≤5 mg/kg | |
| Zn: ≤5 mg/kg | |
| form | powder or crystals |
| ign. residue (900 °C) | ≤0.05% |
| impurities | insoluble matter, passes filter test |
| ≤0.2% water | |
| InChI | 1S/C3H4N2/c1-2-5-3-4-1/h1 |
| InChI key | RAXXELZNTBOGNW-UHFFFAOYSA |
| mp | 88-91 °C (lit.) |
| pH | 9.5-11.0 (25 °C, 0.1 M in H2O) |
| pKa (25 °C) | 6.95 |
| product line | BioUltra |
| Quality Level | 200  |
| SMILES string | c1c[nH]cn1 |
| solubility | H2O: 0.1 M at 20 °C, clear, colorless |
| UV absorption | λ: 260 nm Amax: 0.10 |
| λ: 280 nm Amax: 0.10 | |
| vapor pressure | <1 mmHg ( 20 °C) |
| Application: | Excellent for buffers in the range of pH 6.2-7.8 |
| Application: | Imidazole can be used to prepare buffers in the pH range of 6.2-7.8 at 25°C. It is recommended as a component of a buffer for assay of horseradish peroxidase and as chelator for the binding of various divalent cations. Imidazole can be used for the elution of histidine containing proteins from divalent cation resins (Sigma P6611, HisSelect.-HC Nickel affinity gel) and can also be used in reverse staining of SDS-PAGE gels for detection of proteins. |
| Other Notes: | Buffer for assay of horseradish peroxidase |
| Symbol |    GHS05,GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H302 - H314 - H360D |
| Precautionary statements | P260 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 |
| Hazard Codes | T |
| Risk Statements | 61-22-34 |
| Safety Statements | 53-26-36/37/39-45 |
| RIDADR | UN 3263 8 / PGIII |
| WGK Germany | WGK 2 |
| Flash Point(F) | 293.0 °F - closed cup |
| Flash Point(C) | 145 °C - closed cup |
| Purity | ≥99.5% (GC) |
| bp | 256 °C (lit.) |
| mp | 88-91 °C (lit.) |
| UNSPSC | 12352005 |