Immersion oil
SIGMA/56822 - for microscopy
Synonym: immersion medium for light microscopy
MDL Number: MFCD00131369
Product Type: Chemical
| application(s) | hematology histology |
| biological source | synthetic |
| density | 1.025 g/mL at 20 °C |
| form | liquid |
| grade | for microscopy |
| Quality Level | 100  |
| refractive index | n |
| storage temp. | room temp |
| viscosity | 100-120 mPa.s(20 °C) |
| Application: | Immersion oil is used for high-resolution (1000X) light microscopy work in conjunction with an oil immersion objective lens to optimize microscopic examinations of histological, cytological, hematological, and bacterial specimen material after it has been fixed, embedded, stained, or counterstained, and mounted. |
| General description: | Immersion oil is a clear, viscous liquid with optimized refractive properties, specifically modified to approximate the refractive index of glass as closely as possible. It is used in conjunction with an objective lens to enhance the resolving power. |
| Principle: | Immersion oil is a clear, viscous liquid with optimized refractive properties, specifically modified to closely approximate the refractive index (RI) of glass (ne = 1.5). It is applied dropwise to stained and mounted or non-mounted specimen material to form a clear film between the specimen and the microscope lens to eliminate the deflection of incident light and thus substantially enhance the optical efficiency of the lens. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H315 - H319 - H335 |
| Precautionary statements | P302 + P352 - P305 + P351 + P338 |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 26-36 |
| RIDADR | UN 3082 9 / PGIII |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Density | 1.025 g/mL at 20 °C |
| Refractive Index | n |
| Storage Temp. | room temp |
| UNSPSC | 12352200 |