Immersion oil
SIGMA/56822 - suitable for microscopy
Synonym: oil immersion medium for light microscopy
MDL Number: MFCD00131369
Product Type: Chemical
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| application(s) | hematology histology |
| biological source | synthetic |
| density | 1.025 g/mL at 20 °C |
| form | liquid |
| Quality Level | 100  |
| refractive index | n |
| storage temp. | room temp |
| suitability | suitable for microscopy |
| viscosity | 100-120 mPa.s(20 °C) |
| Application: | Immersion oil is used for high-resolution (1000X) light microscopy work in conjunction with an oil immersion objective lens to optimize microscopic examinations of histological, cytological, hematological, and bacterial specimen material after it has been fixed, embedded, stained, or counterstained, and mounted. |
| Biochem/physiol Actions: | Immersion oil is applied dropwise to stained and mounted or non-mounted specimen material to form a clear film between the specimen and the microscope lens to eliminate the deflection of incident light and thus substantially enhance the optical efficiency of the lens. |
| Features and Benefits: | • Immersion oil for general use in light microscopy. • Excellent, defined refractive index eliminates refractive distortion. • Engineered for optimal viscosity and homogeneity. • Good UV transmission and low residual fluorescence. |
| General description: | Immersion oil is a clear, viscous liquid with optimized refractive properties, specifically modified to closely approximate the refractive index (RI) of glass (ne = 1.5). It is used in conjunction with an objective lens to enhance the resolving power. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H315 - H319 - H335 |
| Precautionary statements | P302 + P352 - P305 + P351 + P338 |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 26-36 |
| RIDADR | UN 3082 9 / PGIII |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Density | 1.025 g/mL at 20 °C |
| Refractive Index | n |
| Storage Temp. | room temp |
| UNSPSC | 12171500 |