Indigo carmine
SIGMA/57000 - for microscopy (Bact., Hist.), indicator (pH 11.5-14.0)
Synonym: Acid Blue 74; Indigo-5,5′-disulfonic acid disodium salt; Indigocarmine
CAS Number: 860-22-0
Empirical Formula (Hill Notation): C16H8N2Na2O8S2
Molecular Weight: 466.35
EC Number: 212-728-8
MDL Number: MFCD00005723
Linear Formula: C16H8N2Na2O8S2
Product Type: Chemical
| εmax | ≥350 at 603-613 nm |
| application(s) | diagnostic assay manufacturing hematology histology |
| density | 0.71 g/cm3 at 29 °C |
| form | powder |
| grade | for microscopy (Bact., Hist.) |
| indicator (pH 11.5-14.0) | |
| InChI | 1S/C16H10N2O8S2.2Na/c19-1 |
| InChI key | KHLVKKOJDHCJMG-QDBORUFSSA |
| loss | ≤5% loss on drying |
| mp | >340 °C |
| pH | 3.87 (30 °C, 7.14 g/L) |
| Quality Level | 100  |
| SMILES string | [Na+].[Na+].[O-]S(=O)(=O) |
| solubility | methanol: 0.01 g/10 mL, blue to very deep blue |
| storage temp. | room temp |
| Application: | Indigo carmine has been used in agarose plates to study behavioral feeding assays in Drosophila larvae. It has also been used for macroscopic assessment of intestinal adenomatous polyps isolated from mice. |
| Biochem/physiol Actions: | Indigo carmine is a contrast dye which helps in highlighting mucosal changes. It accumulates in crevices, cysts and foveolae and stains the tissue blue. The abnormal tissue remains translucent pink. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
| Hazard Codes | Xn |
| Risk Statements | 22 |
| Safety Statements | 22-24/25 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| mp | >340 °C |
| Density | 0.71 g/cm3 at 29 °C |
| Storage Temp. | room temp |
| Colour Index Number | 73015 |
| UNSPSC | 12171500 |