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Lactic Dehydrogenase, recombinant from E. coli

SIGMA/59747 - ≥90 U/mg

Synonym: (S)-Lactate: NAD+ oxidoreductase; L-Lactate Dehydrogenase; Lactate

CAS Number: 9001-60-9
EC Number: 232-617-8
MDL Number: MFCD00131466
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-59747-25MG 25 mg
 $278.00
1/EA		 Add To Favorites
45-59747-100MG 100 mg
 $869.00
1/EA		 Add To Favorites
form powder
Quality Level 100 
recombinant expressed in E. coli
specific activity ≥90 U/mg
storage temp. −20°C
Application: Lactic Dehydrogenase, recombinant from E. coli has been used:
• in lactate dehydrogenase (LDH) and malate dehydrogenase 1 (MDH1)assays and cross-linking assays
• to prepare assay buffer to measure pyruvate kinase (PYK) by coupled assay
• in in vitro DltC D-alanylation assay
Biochem/physiol Actions: L-lactic dehydrogenase catalyzes the conversion of L-lactate into L-pyruvate while reducing NAD+ to NADH and H+.
Biochem/physiol Actions: Conversion of L-lactate into L-pyruvate is crucial in hypoxic and anaerobic conditions, especially when synthesis of adenosine triphosphate (ATP) by oxidative phosphorylation is interrupted.
General description: LDH (lactic dehydrogenase), a glycolytic enzyme, particularly present in skeletal muscle, heart, liver, kidneys, brain, lungs and red blood cells. It has five isoenzyme forms. LDH possess a tetrameric structure.
Unit Definition: One unit corresponds to the amount of enzyme which reduces 1 μmol pyruvate per minute at pH 7.4 and 25°C (NADH as cofactor)
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
activity specific activity: ≥90 U/mg
Storage Temp. −20°C
Enzyme Commission (EC) Number 1.1.1.27   ( BRENDA  | IUBMB  )
UNSPSC 12352204

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