Lactic Dehydrogenase, recombinant from E. coli

SIGMA/59747 - ≥90 U/mg

Synonym: (S)-Lactate: NAD⁺ oxidoreductase; L-Lactate Dehydrogenase; Lactate

CAS Number: 9001-60-9
EC Number: 232-617-8
MDL Number: MFCD00131466
Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-59747-25MG	25 mg	$308.00 1/EA		Add To Favorites
45-59747-100MG	100 mg	$959.00 1/EA		Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

form	powder
Quality Level	100
recombinant	expressed in E. coli
specific activity	≥90 U/mg
storage temp.	−20°C

Application:	Lactic Dehydrogenase, recombinant from E. coli has been used: • in lactate dehydrogenase (LDH) and malate dehydrogenase 1 (MDH1)assays and cross-linking assays • to prepare assay buffer to measure pyruvate kinase (PYK) by coupled assay • in in vitro DltC D-alanylation assay
Biochem/physiol Actions:	L-lactic dehydrogenase catalyzes the conversion of L-lactate into L-pyruvate while reducing NAD⁺ to NADH and H⁺.
Biochem/physiol Actions:	Conversion of L-lactate into L-pyruvate is crucial in hypoxic and anaerobic conditions, especially when synthesis of adenosine triphosphate (ATP) by oxidative phosphorylation is interrupted.
General description:	LDH (lactic dehydrogenase), a glycolytic enzyme, particularly present in skeletal muscle, heart, liver, kidneys, brain, lungs and red blood cells. It has five isoenzyme forms. LDH possess a tetrameric structure.
Other Notes:	One unit corresponds to the amount of enzyme which reduces 1 μmol pyruvate per minute at pH 7.4 and 25°C (NADH as cofactor)

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

activity	specific activity: ≥90 U/mg
Storage Temp.	−20°C
Enzyme Commission (EC) Number	1.1.1.27 ( BRENDA \| IUBMB )
UNSPSC	12352204

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