Chlorophenol Red-β-D-galactopyranoside

SIGMA/59767 - ≥90% (HPLC)

Synonym: CPRG; Chlorophenol Red-β-D-galactoside

CAS Number: 99792-79-7
Empirical Formula (Hill Notation): C₂₅H₂₂Cl₂O₁₀S
Molecular Weight: 585.41
MDL Number: MFCD05863726
Linear Formula: C₂₅H₂₂Cl₂O₁₀S
Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-59767-25MG-F	25 mg	$58.70 1/EA		Add To Favorites
45-59767-100MG-F	100 mg	$172.00 1/EA		Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

assay	≥90% (HPLC)
form	powder or crystals
InChI	1S/C25H22Cl2O10S/c26-15-9-12(5-7-17(15)29)21(14-3-1-2-4-20(14)38(33,34)35)13-6-8-18(16(27)10-13)36-25-24(32)23(31)22(30)19(11-28)37-25/h1-10,19,22-25,28,30-32H,11H2,(H,33,34,35)/b21-12-/t19-,22+,23+,24-,25-/m1/s1
InChI key	YOUWVNCQJOMMEU-AETRGLENSA-N
SMILES string	OC[C@H]1O[C@@H](Oc2ccc(cc2Cl)C(=C3/C=CC(=O)C(Cl)=C3)c4ccccc4S(O)(=O)=O)[C@H](O)[C@@H](O)[C@H]1O
solubility	water: 20 mg/mL, clear, orange to very deep red
storage temp.	−20°C

Application:	Chlorophenol Red-β-D-galactopyranoside has been used as a substrate for β-galactosidase (β-gal): • in paper-based cell-free protein expression system for target RNA detection • in colorimetric detection of multiple clinically relevant mutations using a lacZ output gene • in colorimetric assay for bacteria quantification
General description:	Chlorophenol red-β-D-galactopyranoside (CPRG) is a long-wavelength yellow dye commonly used for colorimetric assays. It serves as a substrate for β-galactosidase (β-gal) which hydrolyzes CPRG to form chlorophenol red (purple colored). This product is quantified by measuring its absorbance at 570 nm. CPRG is used to identify fecal coliform bacteria. It is about 10 times more sensitive than other chromogenic agents such as o-nitrophenyl-β-galactoside (ONPG). CPRG also has a high rapid turnover.
Other Notes:	Measures the expression levels of β-galactosidase in transfected cells. The lacZ gene is a popular reporter gene in transfection reactions because its product, β-galactosidase, is a very stable enzyme.
Packaging:	25, 100 mg in poly bottle

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥90% (HPLC)
Storage Temp.	−20°C
UNSPSC	12352204

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