Nuclear Fast Red
SIGMA/60700 - for microscopy (Bot., Hist.)
Synonym: 4-
CAS Number: 6409-77-4
Empirical Formula (Hill Notation): C14H8NNaO7S
Molecular Weight: 357.27
EC Number: 229-088-0
MDL Number: MFCD00078493
Linear Formula: C14H8NNaO7S
Product Type: Chemical
| εmax | ≥275 at 550-560 nm in DMSO |
| ≥300 at 516-526 nm in DMSO | |
| application(s) | diagnostic assay manufacturing hematology histology |
| form | powder |
| grade | for microscopy (Bot., Hist.) |
| InChI | 1S/C14H9NO7S.Na/c15-9-7-8 |
| InChI key | IFSXZLJQEKGQAF-UHFFFAOYSA |
| Quality Level | 100  |
| SMILES string | [Na+].Nc1c(O)c(c(O)c2C(=O |
| solubility | methanol: water (1:1): 0.01 g/10 mL, red |
| storage temp. | room temp |
| Application: | Nuclear fast red has been used for the nuclear staining of PB (Prussian blue) positive cells in the rostral sub-epidermis and upper beak coronal region isolated from pigeons.Recent applications include:• staining sperm and spermatids in semen • identifying areas for PCR analysis in paraffin-embedded tissues • nuclear counterstaining following immunostaining and silver staining |
| Application: | Used as a biological stain |
| Biochem/physiol Actions: | Nuclear fast red dye has anthraquinonic nature and is used either alone or in combination with other dyes. It is mainly used for staining of nuclei. |
| General description: | Nuclear Fast Red (C.l. 60760) is a substituted hydroxy anthraquinone chromophore that can chelate metal ions. It is also known as kernechtrot and 4-Amino-9,10-dihydro-1,3- |
| Legal Information: | CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Principle: | Nuclear fast red can be used for simple nuclear staining. It is an acidic dyebelonging to the group of anthraquinone dyes. Aluminum sulfate, functioning as a mordant, is added to form a color lake that enables red nuclear staining. Nuclear fast red-aluminum sulfate is used for counterstaining in histochemicalreactions, e. g. in Berlin blue staining for detection of iron or in silver staining. Nuclear fast red for microscopy Certistain® can also be used fornuclear staining with alcian blue. |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 26-36 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| Colour Index Number | 60760 |
| UNSPSC | 12171500 |