Nuclear Fast Red

SIGMA/60700 - suitable for microscopy

Synonym: 4-Amino-9,10-dihydro-1,3-dihydroxy-9,10-dioxo-2-anthracenesulfonic acid sodium salt

CAS Number: 6409-77-4
Empirical Formula (Hill Notation): C₁₄H₈NNaO₇S
Molecular Weight: 357.27
EC Number: 229-088-0
MDL Number: MFCD00078493
Linear Formula: C₁₄H₈NNaO₇S
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-60700-5G	5 g	$167.00 1/EA	Add To Favorites
45-60700-25G	25 g	$475.00 1/EA	Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

ε_max	≥275 at 550-560 nm in DMSO
	≥300 at 516-526 nm in DMSO
application(s)	diagnostic assay manufacturing hematology histology
form	powder
InChI	1S/C14H9NO7S.Na/c15-9-7-8(12(18)14(13(9)19)23(20,21)22)11(17)6-4-2-1-3-5(6)10(7)16;/h1-4,18-19H,15H2,(H,20,21,22);/q;+1/p-1
InChI key	IFSXZLJQEKGQAF-UHFFFAOYSA-M
Quality Level	100
SMILES string	[Na+].Nc1c(O)c(c(O)c2C(=O)c3ccccc3C(=O)c12)S([O-])(=O)=O
solubility	methanol: water (1:1): 0.01 g/10 mL, red
storage temp.	room temp
suitability	suitable for microscopy
technique(s)	microbe id \| staining: suitable

Application:	Nuclear fast red has been used for the nuclear staining of PB (Prussian blue) positive cells in the rostral sub-epidermis and upper beak coronal region isolated from pigeons.Recent applications include:• staining sperm and spermatids in semen • identifying areas for PCR analysis in paraffin-embedded tissues • nuclear counterstaining following immunostaining and silver staining
Application:	Used as a biological stain
Biochem/physiol Actions:	Nuclear fast red can be used for simple nuclear staining. It is an acidic dyebelonging to the group of anthraquinone dyes. Aluminum sulfate, functioning as a mordant, is added to form a color lake that enables red nuclear staining. Nuclear fast red-aluminum sulfate is used for counterstaining in histochemicalreactions, e. g. in Berlin blue staining for detection of iron or in silver staining. Nuclear fast red for microscopy Certistain^® can also be used fornuclear staining with alcian blue.
Biochem/physiol Actions:	Nuclear fast red dye has anthraquinonic nature and is used either alone or in combination with other dyes. It is mainly used for staining of nuclei.
General description:	Nuclear Fast Red (C.l. 60760) is a substituted hydroxy anthraquinone chromophore that can chelate metal ions. It is also known as kernechtrot and 4-Amino-9,10-dihydro-1,3-dihydroxy-9,10-dioxo-2-anthracenesulfonic acid sodium salt. It is used for human-medical cell diagnosis and serves the purpose of the histological investigation of sample material of human origin. It is a dry staining dye that makes target structures (by fixing, wherenecessary embedding, staining with the above nuclear fast red solution, counterstaining, mounting) in human-histological specimen materials, for examplehistological sections of e. g. the kidney, evaluable for diagnostic purposes.
Legal Information:	CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Hazard Codes	Xi
Risk Statements	36/37/38
Safety Statements	26-36
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Storage Temp.	room temp
Colour Index Number	60760
UNSPSC	12171500

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