Penicillinase from Bacillus cereus
SIGMA/61305 - lyophilized, powder, white, ~13 U/mg
Synonym: β-Lactamase I from Bacillus cereus; β-Lactamase II; Cephalosporinase; Penicillin amido-β-lactam hydrolase
CAS Number: 9073-60-3
MDL Number: MFCD00131819
Product Type: Chemical
application(s) | diagnostic assay manufacturing |
biological source | Bacillus sp. (Bacillus cereus) |
color | white |
foreign activity | β-lactamase II ≤2% |
form | powder |
mol wt | Mr ~30000 |
quality | lyophilized |
Quality Level | 100 |
specific activity | ~13 U/mg |
storage temp. | −20°C |
suitability | suitable for component for culture media |
technique(s) | activity assay: suitable |
Application: | Penicillinase from Bacillus cereus may be used in the preparation of biosensor for amperometric detection of hydrolysis of penicillin. |
Application: | Used in the production of penicillin. |
Biochem/physiol Actions: | Penicillinase specifically catalyzes the hydrolysis of β-lactam ring of penicillin. |
General description: | Penicillinase from Bacillus cereus is both an extracellular and cell-bound enzyme. It has a single polypeptide chain and has a molecular weight of 31 kDa. It lacks cysteine in the protein sequence. |
Other Notes: | Used to destroy β-lactam antibiotics in body fluids or culture media. For the determination of penicillin; In the coupled enzyme assay for isopenicillin N synthetase; ELISA of estradiol; Penicillinase optodes |
Packaging: | Bottomless glass bottle. Contents are inside inserted fused cone. |
Unit Definition: | One unit will hydrolyze 1.0 μmole of indicated substrate per min at pH 7.0 at 25 °C. Sold as benzylpenicillin units. This International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units. |
Symbol | GHS08 |
Signal word | Danger |
Hazard statements | H317 - H334 |
Precautionary statements | P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313 |
Hazard Codes | Xn |
Risk Statements | 42/43 |
Safety Statements | 22-36 |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
activity | specific activity: ~13 U/mg |
Storage Temp. | −20°C |
Enzyme Commission (EC) Number | 3.5.2.6 ( BRENDA | IUBMB ) |
UNSPSC | 12352204 |