Lipase B Candida antarctica, recombinant from Aspergillus oryzae
SIGMA/62288 - powder, beige, ~9 U/mg
CAS Number: 9001-62-1
EC Number: 232-619-9
MDL Number: MFCD00131509
Product Type: Chemical
| color | beige |
| form | powder |
| InChI | 1S/C11H9N3O2.Na/c15-8-4-5 |
| InChI key | QWZUIMCIEOCSJF-CHHCPSLASA |
| mol wt | 33 kDa |
| Quality Level | 100  |
| recombinant | expressed in Aspergillus oryzae |
| specific activity | ~9 U/mg |
| storage temp. | 2-8°C |
| Application: | Lipase B Candida antarctica, recombinant from Aspergillus oryzae has been used: • as a standard to characterize the enzymatic properties of D5-CalB • as an efficient biocatalyst to start the reaction to obtain (R)-ester via esterification of racemic secondary alcohol • to investigate a “green” recycling route for polybutylene succinate (PBS) based on reactive extrusion • to compare the esterification yield with adsorbed CaLB (aCaLB) and covalently immobilized CaLB (cCaLB) |
| Application: | Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel. |
| Biochem/physiol Actions: | Lipase B from Candida antarctica has been shown to be an effective catalyst for the synthesis of esters of ethyl |
| Biochem/physiol Actions: | Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids. Candida antarctica lipase B (CALB) possesses wide substrate specificity, high activity and high enantioselectivity, hence it is considered as a major enzyme in biotechnology. It also has the capability to perform in aqueous and non-aqueous reaction environments. CALB is used in transesterification, kinetic resolution and polymerization reactions. |
| General description: | Candida antarctica lipase B (CalB) is structurally similar to several other lipases and has a flexible lid. It is made up of 317 amino acids and has a molecular weight of 33 kDa. Lipase B is a member of the alpha/beta hydrolase-fold family. |
| Unit Definition: | 1 U corresponds to the amount of enzyme which liberates 1 μmol butyric acid per minute at pH 8.0 and 40°C (tributyrin, Cat. No. 91010, as substrate) |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ~9 U/mg |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.1.1.3 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |