Lipase from Rhizopus oryzae
SIGMA/62305 - powder (fine), ~10 U/mg
Synonym: Lipase from Rhizopus arrhizus; Triacylglycerol acylhydrolase; Triacylglycerol lipase
CAS Number: 9001-62-1
EC Number: 232-619-9
MDL Number: MFCD00131509
Product Type: Chemical
| form | powder (fine) |
| InChI | 1S/C11H9N3O2.Na/c15-8-4-5 |
| InChI key | QWZUIMCIEOCSJF-CHHCPSLASA |
| mol wt | Mr ~43000 |
| Quality Level | 100  |
| specific activity | ~10 U/mg |
| storage temp. | 2-8°C |
| Application: | Lipase from Rhizopus oryzae has been used: • to test its effect on 1,2-diolein synthesis and triolein ethanolysis • for immobilization on graphene oxide support for biocatalysis studies • to digest triglycerides (TAG) from Chlamydomonas reinhardtii and S. cerevisiae |
| Application: | Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel. |
| Biochem/physiol Actions: | Lipase from Rhizopus oryzae (ROL) acts as a catalyst for the enzymatic biosynthesis of polyglycerol polyricinoleate through a reversal of hydrolysis. ROL is useful in the industrial production of structured lipids due to its 1,3-regiospecificity functionality. |
| Biochem/physiol Actions: | Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate). Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids. |
| General description: | Lipase from Rhizopus oryzae (ROL) comprises an oxyanion hole, four N-glycosylation sites, and an active site region. It possesses N-terminal presequence and prosequence. |
| Other Notes: | Catalyst for the interesterification of oils and fats; For removal of interfering triglycerides in the electroimmunoassay of apolipoprotein B; Racemic epoxy ester resolution through enantioselective enzymatic hydrolysis |
| Other Notes: | Note: When triacetin is used as substrate, the pH is 7.4. Incubation time: 60 minutes. |
| Unit Definition: | 1 U corresponds to the amount of enzyme which liberates 1 μmol of butyric acid per minute at pH 8.0 and 40°C (tributyrin, Cat. No. 91010 as substrate) 5000 U as described above are equivalent to ~1 U using triolein, Cat. No. 62314 as substrate, at pH 8.0 and 40°C |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ~10 U/mg |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.1.1.3 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |