Lipase from Rhizopus niveus
SIGMA/62310 - powder (fine), ≥1.5 U/mg
Synonym: Triacylglycerol acylhydrolase; Triacylglycerol lipase
CAS Number: 9001-62-1
EC Number: 232-619-9
MDL Number: MFCD00131509
Product Type: Chemical
| biological source | fungus (Rhizopus niveus) |
| form | powder (fine) |
| InChI | 1S/C11H9N3O2.Na/c15-8-4-5 |
| InChI key | QWZUIMCIEOCSJF-CHHCPSLASA |
| mol wt | Mr ~83000 |
| Quality Level | 100  |
| specific activity | ≥1.5 U/mg |
| storage temp. | 2-8°C |
| Application: | Lipase from Rhizopus niveus has been used to determine the positional distribution of fatty acids within triacylglycerol by hydrolysis of the acyl ester linkage at C-1 and C-3. It has also been used to induce intragastric lipolysis and study its effects on felodipine (a medication) release from a hydrophilic, extended release tablet. |
| Biochem/physiol Actions: | Lipase from Rhizopus niveus is specifically used to produce cocoa butter substitutes. |
| Biochem/physiol Actions: | Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate). |
| General description: | Lipases hydrolyze ester bonds of triacylglycerols and in some conditions, catalyze the synthesis of ester bonds by transesterification. Lipase from Rhizopus niveus exists as a single polypeptide chain with a molecular mass of about 30-34kDa. |
| Other Notes: | Note: When triacetin is used as substrate, the pH is 7.4. Incubation time: 60 minutes. |
| Other Notes: | Substrate specificity: preferentially hydrolyzes the 1- and 3-positions(middle to long fatty acids); optimum pH 5.0-7.0; optimum temperature 30-45°C; Selective enzymatic removal of protecting functions (C-terminal COOH function) |
| Unit Definition: | 1 U corresponds to the amount of enzyme which liberates 1 μmol fatty acid from a triglyceride per minute at pH 7.7 and 40°C (olive oil as substrate)]; 300 U as described above are equivalent to ~1 U using triolein, Cat. No. 62314, at pH 8.0 and 40°C as substrate. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥1.5 U/mg |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.1.1.3 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |