DL-Dithiothreitol solution

SIGMA/646563 - 1 M in H₂O

Synonym: (±)-threo-1,4-Dimercapto-2,3-butanediol solution; DTT

CAS Number: 3483-12-3
Empirical Formula (Hill Notation): C₄H₁₀O₂S₂
Molecular Weight: 154.25
MDL Number: MFCD00004877
Linear Formula: C₄H₁₀O₂S₂
Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-646563-10X.5ML	0.5 mL	$107.00 10/EA		Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

concentration	1 M in H₂O
density	1.035 g/mL at 25 °C
	1.04 g/mL at 20 °C
form	liquid
InChI	1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
InChI key	VHJLVAABSRFDPM-QWWZWVQMSA-N
Quality Level	100
reaction suitability	reagent type: reductant
SMILES string	O[C@H](CS)[C@H](O)CS

Application:	DL-Dithiothreitol solution has been used: • in western blot analysis • as a supplement in lysis buffer to study its effects on sperm lysis • as a component of cold homogenization buffer, and chilled wash buffer for the isolation of nuclei from frozen rat tissues • in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), chromatography, modification of cysteine-containing compounds
Features and Benefits:	Low toxicity and reduced odor compared to other thiol compounds.
General description:	Dithiothreitol (DTT) is used in proteomics applications to maintain sulfhydryl (-SH) groups in the reduced state and for quantitative reduction of disulfide (-S-S-) groups. It is a frequently used reagent in buffers due to its ability to reduce the oxidation state of a protein sample and preserves enzymatic activity. DTT is oxidized to the cyclic disulfide during the reduction of other disulfides in the solution.
General description:	Dithiothreitol is an effective reducing agent for protein analysis. The sealed-ampule format is convenient and easy to use when preparing samples for electrophoresis or other applications and avoids waste and loss of potency.
Packaging:	10×0.5 mL in ampule

Symbol	GHS05
Signal word	Danger
Hazard statements	H315 - H318
Precautionary statements	P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P362 + P364
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Density	1.04 g/mL at 20 °C; 1.035 g/mL at 25 °C
UNSPSC	12161504

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