Imidazole buffer Solution
SIGMA/68268 - BioUltra, 1 M in H2O
Synonym: Imidazole solution; Glyoxaline solution
CAS Number: 288-32-4
Empirical Formula (Hill Notation): C3H4N2
Molecular Weight: 68.08
MDL Number: MFCD00005183
Linear Formula: C3H4N2
Product Type: Chemical
| concentration | 1 M in H2O |
| density | 1.01 g/mL at 20 °C |
| form | liquid |
| impurities | DNases, none detected |
| insoluble matter, passes filter test | |
| phosphatases, none detected | |
| proteases, none detected | |
| RNases, none detected | |
| InChI | 1S/C3H4N2/c1-2-5-3-4-1/h1 |
| InChI key | RAXXELZNTBOGNW-UHFFFAOYSA |
| pH | 9.5±0.5 |
| product line | BioUltra |
| Quality Level | 200  |
| SMILES string | c1c[nH]cn1 |
| Application: | Imidazole buffer solution has been used as a component: • of calmodulin-binding buffer 1 (CBB1) for the tandem affinity purification (TAP) of TAP-tagged proteins together with co-purifying proteins • of lysis buffer, and SDS loading buffer for His-ubiquitin pull-down assays • of imidazole wash and elution buffer for protein purification |
| General description: | Imidazole is used as a buffer in the pH range of 6.2-7.8 at 25°C. It is recommended as a component of a buffer for the assay of horseradish peroxidase and as a chelator for the binding of various divalent cations. Imidazole aids the elution of histidine-containing proteins from divalent cation resins (Sigma P6611, HisSelect.-HC Nickel affinity gel) and can also be used in reverse staining of SDS-PAGE gels for the detection of proteins. |
| Symbol |   GHS05,GHS08 |
| Signal word | Danger |
| Hazard statements | H314 - H360D |
| Precautionary statements | P201 - P280 - P301 + P330 + P331 - P303 + P361 + P353 - P305 + P351 + P338 + P310 - P308 + P313 |
| Hazard Codes | T |
| Risk Statements | 61-36/38 |
| Safety Statements | 53-26-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Density | 1.01 g/mL at 20 °C |
| UNSPSC | 12161700 |