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Fluorescent Red Mega 485 NHS-ester

SIGMA/68360 - BioReagent, suitable for fluorescence, ≥90% (coupling to amines)

CAS Number: 890317-36-9
Empirical Formula (Hill Notation): C29H33N3O9S
Molecular Weight: 599.65
MDL Number: MFCD06798200
Linear Formula: C29H33N3O9S
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-68360-1MG-F 1 mg
 $459.00
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assay ≥90% (coupling to amines)
fluorescence λex 482 nm; λem 559 nm in 0.1 M phosphate pH 7.0
form powder
InChI 1S/C29H33N3O9S/c1-2-31(15-5-3-4-7-28(35)41-32-26(33)10-11-27(32)34)23-9-8-22-19-24(29(36)40-25(22)20-23)21-12-16-30(17-13-21)14-6-18-42(37,38)39/h8-9,12-13,16-17,19-20H,2-7,10-11,14-15,18H2,1H3
InChI key VKRRBXLGPVGMGA-UHFFFAOYSA-N
product line BioReagent
Quality Level 100 
SMILES string CCN(CCCCCC(=O)ON1C(=O)CCC1=O)c2ccc3C=C(C(=O)Oc3c2)c4cc[n+](CCCS([O-])(=O)=O)cc4
storage temp. 2-8°C
suitability suitable for fluorescence
Application: Fluorescent Red Mega 485 NHS-ester is an amine conjugating fluorescent biolabel that can be used for covalent coupling to proteins and other biomolecules containing primary amino groups.Fluorescent red Mega 485 and other Mega dyes are specifically designed for multicolor techniques. It is characterized by an extremely large stoke′s shift between excitation and emission maxima. This dye is especially well suited for excitation by argon lasers or other short wavelength excitation light. This means that it can be excited with the same light source or filter arrangement than fluorescein and other widely used dyes as well as other Mega dye. Due to the different emission wavelength several different labels can be detected independently in the same experiment. Due to the small size of the Mega-labels and the direct conversion of excitation light into emission without using a FRET mechanism we foresee a variety of possible applications including DNA sequencing and FISH microscopy.
Symbol GHS05  GHS05
Signal word Danger
Hazard statements H318
Precautionary statements P280 - P305 + P351 + P338
Hazard Codes Xi
Risk Statements 41
Safety Statements 26-39
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥90% (coupling to amines)
Storage Temp. 2-8°C
UNSPSC 12352108

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