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4-Methylumbelliferyl N-acetyl-β-D-glucosaminide

SIGMA/69585 - suitable for fluorescence, ≥99.0% (TLC)

Synonym: 4-Methylumbelliferyl-2-acetamido-2-deoxy-β-D-glucopyranoside

CAS Number: 37067-30-4
Empirical Formula (Hill Notation): C18H21NO8
Molecular Weight: 379.36
EC Number: 253-333-0
MDL Number: MFCD00150977
Linear Formula: C18H21NO8
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-69585-250MG 250 mg
 $372.00
1/EA		 Add To Favorites
45-69585-1G 1 g
 $1240.00
1/EA		 Add To Favorites

 

assay ≥99.0% (TLC)
fluorescence λex 317 nm (pH 10.0)
  λex 365 nm; λem 445 nm in aqueous buffer pH 5.0 (after cleavage by β-N-acetylglucosaminidase)
form powder
impurities ≤0.1% free 4-methylumbelliferone
InChI 1S/C18H21NO8.2H2O/c1-8-5-14(22)26-12-6-10(3-4-11(8)12)25-18-15(19-9(2)21)17(24)16(23)13(7-20)27-18;;/h3-6,13,15-18,20,23-24H,7H2,1-2H3,(H,19,21);2*1H2/t13-,15-,16-,17-,18-;;/m1../s1
InChI key PAVCYMSNMRWMAK-DMYIEBNJSA-N
Quality Level 100 
SMILES string [H]O[H].[H]O[H].CC(=O)N[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1Oc2ccc3C(C)=CC(=O)Oc3c2
solubility DMF: 20 mg/mL, clear, colorless
storage temp. −20°C
suitability suitable for fluorescence
Application: 4-Methylumbelliferyl N-acetyl-β-D-glucosaminide has been used as a fluorogenic substrate in the acidic chitinase activity assay. It has also been used to measure the total activity of β-N-acetylhexosaminidase (NAGase) in water sample filtrates.
Biochem/physiol Actions: 4-Methylumbelliferyl-N-acetyl-β-D-glucosaminide (4-MUF-NAG) is a synthetic uncharged fluorogenic substrate for hexosaminidases. It consists of chitin monomer (N-acetyl-β-D-glucosaminide) and 4-methylumbelliferone (7-hydroxy-4-methylcoumarin) (4-MUF). 4-MUF-NAG is used for the detection and estimation of fungal growth by measuring the β-N-acetylhexosaminidase (NAGase) activity.
Other Notes: 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research 
Other Notes: Assay of β-N-acetylhexosaminidase.
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥99.0% (TLC)
Storage Temp. −20°C
UNSPSC 12352204

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