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2′-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid sodium salt hydrate

SIGMA/69587 - BioReagent, suitable for fluorescence, ≥96.5% (HPLC)

Synonym: 4-MUNANA; 4-Methylumbelliferyl-N-acetyl-α-D-neuraminic acid sodium salt hydrate; 4-Methylumbelliferyl-N-acetyl-α-D-neuraminic acid sodium salt hydrate

CAS Number: 76204-02-9 (anhydrous)
Empirical Formula (Hill Notation): C21H24NNaO11 · xH2O
Molecular Weight: 489.41 (anhydrous basis)
MDL Number: MFCD00057309
Linear Formula: C21H24NNaO11 · xH2O
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-69587-5MG 5 mg
 $358.00
1/EA		 Add To Favorites
45-69587-25MG 25 mg
 $1460.00
1/EA		 Add To Favorites

 

assay ≥96.5% (HPLC)
color white to faint yellow
composition free 4-methylumbelliferone, ≤0.5%
  water, ≤10%
fluorescence λex 315 nm; λem 374 nm (pH.7.0)
  λex 365 nm; λem 445 nm (after cleavage by neuraminidase)
form powder
impurities ≤0.5% free 4-methylumbelliferone
  ≤10% water
InChI 1S/C21H25NO11.Na.H2O/c1-9-5-16(27)31-15-6-11(3-4-12(9)15)32-21(20(29)30)7-13(25)17(22-10(2)24)19(33-21)18(28)14(26)8-23;;/h3-6,13-14,17-19,23,25-26,28H,7-8H2,1-2H3,(H,22,24)(H,29,30);;1H2/q;+1;/p-1/t13-,14?,17+,18?,19+,21+;;/m0../s1
InChI key NSQMRVBWXQQIKF-NVRWCLOTSA-M
mol wt 489.41 g/mol (anhydrous basis)
product line BioReagent
SMILES string O.[H]C(O)(CO)[C@]([H])(O)[C@@H]1OC(C[C@H](O)[C@H]1NC(C)=O)(Oc2ccc3C(C)=CC(=O)Oc3c2)C(=O)O[Na]
solubility H2O: 50 mg/mL, clear, very slightly yellow
storage temp. −20°C
suitability suitable for fluorescence
Application: 2′-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid or 4-MUNANA is used as a substrate for the development of a microplate-based assay for neuraminidase inhibitors. The fluorogenic substrate 2ʹ-(4-methylumbelliferyl)-α-D-N-acetylneuraminic acid (4-MUNANA) is used to study the molecular mechanisms of aspirin and celecoxib targeting mammalian neuraminidase-1.
Biochem/physiol Actions: Substrate for fluorometric assay of neuraminidase. Used for fluorescent staining of sialidases in PAGE.
General description: 2′-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid or 4-MUNANA is a synthetic fluorometric substrate that mimics the naturally occurring neuraminidase enzyme substrate. The enzyme-substrate hydrolysis results in the product 4-methylumbelliferone (4-MU), measured fluorometrically.
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥96.5% (HPLC)
Storage Temp. −20°C
UNSPSC 12352106

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