MES monohydrate

SIGMA/69892 - BioXtra, ≥99.0% (T)

Synonym: 2-(4-morpholino)ethanesulfonic acid; 2-morpholinoethanesulfonic acid; Morpholine-4-ethanesulfonic acid hydrate; 2-(N-Morpholino)ethanesulfonic acid; 4-Morpholineethanesulfonic acid monohydrate

CAS Number: 145224-94-8
Empirical Formula (Hill Notation): C₆H₁₃NO₄S · H₂O
Molecular Weight: 213.25
EC Number: 224-632-3
MDL Number: MFCD00149409
Linear Formula: C6H13NO4S · H2O
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-69892-25G	25 g	$73.20 1/EA	Add To Favorites
45-69892-100G	100 g	$225.00 1/EA	Add To Favorites
45-69892-500G	500 g	$796.00 1/EA	Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

anion traces	chloride (Cl^-): ≤50 mg/kg
	sulfate (SO₄^2-): ≤50 mg/kg
application(s)	agriculture diagnostic assay manufacturing general analytical microbiology
assay	≥99.0% (T)
cation traces	Ca: ≤50 mg/kg
	Cd: ≤5 mg/kg
	Co: ≤5 mg/kg
	Cr: ≤5 mg/kg
	Cu: ≤5 mg/kg
	Fe: ≤5 mg/kg
	K: ≤50 mg/kg
	Mg: ≤5 mg/kg
	Mn: ≤5 mg/kg
	Na: ≤50 mg/kg
	Ni: ≤5 mg/kg
	Pb: ≤5 mg/kg
	Zn: ≤5 mg/kg
foreign activity	DNase, none detected
	NICKase, none detected
	protease, none detected
	RNase, none detected
form	powder or crystals
InChI	1S/C6H13NO4S.H2O/c8-12(9,10)6-3-7-1-4-11-5-2-7;/h1-6H2,(H,8,9,10);1H2
InChI key	MIIIXQJBDGSIKL-UHFFFAOYSA-N
mp	>300 °C (lit.)
pKa (25 °C)	6.1
product line	BioXtra
Quality Level	200
SMILES string	O.OS(=O)(=O)CCN1CCOCC1
solubility	H₂O: 0.5 M at 20 °C, clear
useful pH range	5.5-6.7

Application:	MES Monohydrate has been used: • to adjust the pH of the growth medium, facilitating gene expression analyses aimed at understanding the molecular mechanisms • in the fixation and imaging of cellular components, enabling the study of hypotonic stress′s physiological effects on cell structure and function through Super-Resolution and Live-Cell Microscopy • to adjust the pH of the growth medium to 5.5-7.0 in an in vitro cell wall stress assay • as a buffering agent to stabilize enzymatic solutions • as a component of PAGE running buffer
Features and Benefits:	• Suitable for Biochemical and Cell Biology Research • Tested for the presence of anionic and cationic traces • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications • Effective Buffering from pH 5.5-6.7 (25 °C) with a pKa of 6.1 (25 °C)
General description:	MES monohydrate, also known as 2-Morpholinoethanesulfonic acid hydrate, is a buffer used in research applications in biology and biochemistry. MES is a pH buffering compound with a pKa value of 6.15 at 20 ^oC. MES was developed in the 1960s as one of Good′s buffers, which were designed with various criteria in mind, including midrange pKa, maximum water solubility, minimum solubility in all other solvents, and minimal change in pKa with temperature. MES is highly soluble in water and only weakly binds with certain metal ions, including Ca, Mg, Mn, and Cu(II), which makes it a non-coordinating buffer in chemistry involving metal ions. Further, It plays a crucial role in maintaining a stable environment in various applications, including cell culture media, protein-based buffer formulations, and electrophoresis running buffers. MES monohydrate finds extensive use in purifying antibodies, peptides, proteins, blood components, and growth factors. It also stands out as an excellent choice for capillary electrochromatography due to its low ionic mobility, offering a safer alternative to toxic compounds like cacodylate and non-zwitterionic buffers such as citrate and malate. Additionally, MES Monohydrate serves various purposes, including pH adjustment in growth media, stabilizing enzymatic solutions, and as a component of PAGE running buffers in diverse experimental settings.
Other Notes:	Solubility/Stability: MES is soluble in water, giving a clear colourless solution at concentrations of 0.5M or higher. The pH of a solution should be between 2.5 and 5, depending on the concentration. Solutions are stable at 2-8°C for months. Sterilization: Sterilization should be by filteration through 0.2μM filters. Autoclaving is not recommended by any sulfonic acid buffers. If buffers must be nuclease-free, it is best to treat the water, then add the buffer solids after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably. The identity of the yellow breakdown product is unkown.
Other Notes:	For additional information on our range of Biochemicals , please complete this form .
Preparation Note:	A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pKa =±1). Alternatively, volumes of equimolar MES free acid and sodium MES can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare a buffer of a given pH have been published.

Hazard Codes	Xi
Risk Statements	36/37/38
Safety Statements	26-36
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥99.0% (T)
mp	>300 °C (lit.)
UNSPSC	12161700

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