Bromobimane

SIGMA/69898 - BioReagent, suitable for fluorescence, ≥95% (HPCE)

Synonym: Monobromobimane; Monobromobimane

CAS Number: 71418-44-5
Empirical Formula (Hill Notation): C₁₀H₁₁BrN₂O₂
Molecular Weight: 271.11
MDL Number: MFCD00036951
Linear Formula: C₁₀H₁₁BrN₂O₂
Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-69898-25MG	25 mg	$159.00 1/EA		Add To Favorites
45-69898-100MG	100 mg	$509.00 1/EA		Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

assay	≥95% (HPCE)
fluorescence	λ_ex 390 nm; λ_em 478 nm in 0.1 M phosphate pH 7.5 (after derivatization with glutathione)
	λ_ex 398 nm
form	solid
InChI	1S/C10H11BrN2O2/c1-5-7(3)12-8(4-11)6(2)10(15)13(12)9(5)14/h4H2,1-3H3
InChI key	AHEWZZJEDQVLOP-UHFFFAOYSA-N
mp	152-154 °C (lit.)
product line	BioReagent
Quality Level	100
SMILES string	CC1=C(C)C(=O)N2N1C(CBr)=C(C)C2=O
solubility	acetonitrile: soluble
	DMF: soluble
	DMSO: soluble
	methanol: soluble
storage temp.	2-8°C
suitability	suitable for fluorescence

Application:	Bromobimane is used for the determination of thiols by the HPLC method. It is suitable as a pre-column derivatization agent for fluorometric determination of 2,3-dimercaptopropane-1-sulfonic acid and other dithiols. Bromobimane has been used as a fluorescent label in studying oligomycin-sensitive ATPase from beef heart mitochondria.
Features and Benefits:	The benefits of using Bromobimane for the analysis of low molecular weight thiols are: Highly selective.• Rapid reactivity. • Ease of separation of derivatives by reverse HPLC. • Ability to penetrate cells.
General description:	Bromobimane, also called probe for thiols, is a fluorescent reagent activated upon a photolysis reaction. Bromobimane in solution reacts with small thiol groups (e.g., GSH) and with reactive protein thiol groups (e.g., hemoglobin). The reaction of Bromobimane with thiols is of second-order and dependent on pH. And, upon reacting with thiolate, it activates the water-soluble fluorescent product for detection.
Other Notes:	Fluorescent thiol-specific reagent
Packaging:	Bottomless glass bottle. Contents are inside inserted fused cone.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥95% (HPCE)
mp	152-154 °C (lit.)
Storage Temp.	2-8°C
UNSPSC	12352200

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